Chromosome changes in rat embryo cell lines transformed by temperature-sensitive mutants and sheared DNA of herpes simplex virus.

The chromosomes of six rat embryo cell lines transformed with herpes simplex virus (HSV) temperature-sensitive (ts) mutants were examined at different passages of in vitro cultivation. Two cell lines were predominantly diploid, one cell line was hyperdiploid, one cell line was pseudodiploid, and two cell lines were hypotetraploid. In near-diploid cell lines chromosome No. 9 was most frequently involved in chromosome changes. All three cell lines derived from tumors obtained after one transplantation of HSV-transformed cells into baby rats were pseudodiploid, but each had different marker chromosomes. Chromosome No. 15 was involved in the formation of two out of four marker chromosomes. Four cell lines derived from tumors developing after two and three transplantations were hypodiploid and showed large chromosome variation. The occurrence of 25 marker chromosomes in three tumor-derived cell lines resulted in gains in parts from chromosomes No. 2, 6, and 7. One marker chromosome had a homogeneously faintly stained region. Chromosomes No. 2, 3, 7, and 12 were more frequently involved in the formation of marker chromosomes. No chromosome change was found to be specifically associated with HSV-induced transformation of rat cells, but chromosome changes in tumor-derived cell lines may provide selective advantage for survival and autonomous growth in the host animal.