Nussbaum S R, Carr D B, Bergland R M, Kliman B, Fisher J, Reiner B, Kleshinski S, Rosenblatt M
Endocrinology. 1983 May;112(5):1877-9. doi: 10.1210/endo-112-5-1877.
Corticotropin-releasing factor (CRF), recently isolated from sheep hypothalami, has been shown to stimulate secretion of ACTH and beta-endorphin in vitro, and in vivo in rat and man. In previous reports, responses to ovine CRF were studied in heterologous bioassay systems where the ovine sequence was likely to act as a CRF analogue. We administered synthetic ovine CRF to sheep to assess the dynamics of endorphin and cortisol responses. Graded doses of CRF caused a rapid increase in immunoreactive beta-endorphin (iB-E) within 2 min of iv administration, followed by a cortisol response which was maximal 15 min after the iB-E peak. Doses of CRF in excess of 10 micrograms did not increase the magnitude of the peak iB-E response but did prolong the duration of the plasma beta-endorphin rise. Ovine CRF is an extremely potent and rapidly-acting hypothalamic peptide in vivo when assayed in a homologous system.
促肾上腺皮质激素释放因子（CRF），最近从绵羊下丘脑分离出来，已被证明在体外以及在大鼠和人类体内均能刺激促肾上腺皮质激素（ACTH）和β-内啡肽的分泌。在以前的报告中，在异源生物测定系统中研究了对羊CRF的反应，在该系统中羊的序列可能作为CRF类似物起作用。我们给绵羊注射合成的羊CRF以评估内啡肽和皮质醇反应的动态变化。静脉注射不同剂量的CRF后，在2分钟内免疫反应性β-内啡肽（iB-E）迅速增加，随后出现皮质醇反应，在iB-E峰值后15分钟达到最大值。超过10微克的CRF剂量并未增加iB-E峰值反应的幅度，但确实延长了血浆β-内啡肽升高的持续时间。在同源系统中进行测定时，羊CRF在体内是一种极其有效且作用迅速的下丘脑肽。
