Aubin J E, Alders E, Heersche J N
Exp Cell Res. 1983 Feb;143(2):439-50. doi: 10.1016/0014-4827(83)90070-8.
The distributions of microfilaments and microtubules were studied during transient hormone-induced changes in cell shape (retraction-respreading). Two cell types (fibroblasts and bone cells), differentially responsive to parathyroid hormone (PTH) and prostaglandin E2 (PGE2), were analysed. The cytoplasm of fibroblasts retracted in response to PGE2 but not PTH, whereas bone cells could respond to both PGE2 and PTH. Time-lapse photomicrography indicated that the retraction began within minutes of hormone addition, while respreading occurred over longer times, up to 8 h. Affinity-purified actin and tubulin antibodies were used to follow the appearance of microtubules and microfilaments during both the retraction and the respreading phases. Microtubules appeared not to reorganize noticeably, although they were squeezed closer together in cellular pseudopods; no extensive loss or growth was detectable. Microfilaments did alter drastically their appearance and distributions. Soon after hormone addition when earliest detectable cytoplasmic retraction was evident, microfilament bundles appeared to break down. Remaining microfilament bundles consisted of relatively short, non-aligned fragments or aggregates. During respreading, microfilament bundles regrew and realigned throughout the cytoplasm. These data suggest a primary role for microfilaments, but probably not microtubules, in these cell shape changes.
在激素诱导的细胞形状短暂变化（收缩-重新铺展）过程中，对微丝和微管的分布进行了研究。分析了两种对甲状旁腺激素（PTH）和前列腺素E2（PGE2）反应不同的细胞类型（成纤维细胞和骨细胞）。成纤维细胞的细胞质对PGE2有反应而对PTH无反应，而骨细胞对PGE2和PTH都有反应。延时显微摄影表明，收缩在添加激素后几分钟内开始，而重新铺展则需要更长时间，长达8小时。亲和纯化的肌动蛋白和微管蛋白抗体被用于追踪收缩和重新铺展阶段微管和微丝的出现情况。微管似乎没有明显的重新组织，尽管它们在细胞伪足中被挤压得更紧密；没有检测到大量的损失或生长。微丝的外观和分布确实发生了剧烈变化。在添加激素后不久，当最早可检测到的细胞质收缩明显时，微丝束似乎分解了。剩余的微丝束由相对较短、未排列的片段或聚集体组成。在重新铺展过程中，微丝束在整个细胞质中重新生长并重新排列。这些数据表明微丝在这些细胞形状变化中起主要作用，但微管可能不起主要作用。
