Yagawa K, Itoh T, Tomoda A
FEBS Lett. 1983 Apr 18;154(2):383-6. doi: 10.1016/0014-5793(83)80187-2.
Superoxide (O2-) generation of guinea-pig macrophages induced by wheat germ agglutinin (WGA) was suppressed to a great extent by the inhibition of transmethylation with 3'-deazaadenosine. When macrophages were stimulated with phorbor myristate (PMA) instead of WGA, the suppression of O2- generation of macrophages was observed to be slight despite the presence of 3'-deazaadenosine. These results were confirmed under various conditions. Thus the WGA-stimulated O2- generation of macrophages is probably associated with transmethylation, but the PMA-stimulated O2- generation is not. WGA-stimulated O2- generation of macrophages was also inhibited in the presence of dibutyryl cAMP or prostaglandin E2 (PGE2), substances that increase intracellular cAMP, but PMA-stimulated O2- generation was only slightly affected by these compounds. These results suggest that the mechanism for O2- generation of macrophages caused by WGA is different from that for O2- generation caused by PMA.
用3'-脱氮腺苷抑制转甲基作用，可在很大程度上抑制麦胚凝集素（WGA）诱导的豚鼠巨噬细胞超氧阴离子（O2-）生成。当用佛波醇肉豆蔻酸酯（PMA）而非WGA刺激巨噬细胞时，尽管存在3'-脱氮腺苷，巨噬细胞O2-生成的抑制作用仍很轻微。这些结果在各种条件下均得到证实。因此，WGA刺激的巨噬细胞O2-生成可能与转甲基作用有关，而PMA刺激的O2-生成则不然。在存在二丁酰环磷腺苷（cAMP）或前列腺素E2（PGE2）（可增加细胞内cAMP的物质）的情况下，WGA刺激的巨噬细胞O2-生成也受到抑制，但PMA刺激的O2-生成仅受到这些化合物的轻微影响。这些结果表明，WGA引起的巨噬细胞O2-生成机制与PMA引起的O2-生成机制不同。
