Tsuru S, Nomoto K, Aiso S, Ogata T, Zinnaka Y
Int Arch Allergy Appl Immunol. 1983;71(1):88-92.
The gangliosides compose a major portion of the surface membrane structure of the lymphocytes and their expression may relate with functional properties of different lymphocyte subpopulations. In the present study, the binding of the ganglioside GM1 to the human peripheral blood mononuclear cells (PBMC) and their effects on the lymphocyte function were examined. Cholera toxin (CT) was used as an indicator for detection of GM1 on the cell surface. It was shown that the exogenous GM1 binds to normal PBMC and inhibits proliferative responses in vitro by various mitogens (Con A, PHA, PWM). It was also revealed that more than 18 h is required to induce unresponsiveness of lymphocytes by preincubation with GM1, even though GM1 bound very rapidly (10-30 min) to lymphocytes. Some intracellular event may be needed to induce unresponsive state of the lymphocytes by GM1 binding. Moreover, the number of CT binding lymphocytes and the amount of CT bound to each cell showed to increase in the cancer patients. These results suggest that the increase of GM1 in the serum and the lymphocyte surface may be one of the mechanisms of suppressed lymphocyte responsiveness in the various pathological states especially in the cancer patients.
神经节苷脂构成淋巴细胞表面膜结构的主要部分，其表达可能与不同淋巴细胞亚群的功能特性相关。在本研究中，检测了神经节苷脂GM1与人外周血单个核细胞（PBMC）的结合及其对淋巴细胞功能的影响。霍乱毒素（CT）用作检测细胞表面GM1的指标。结果表明，外源性GM1可与正常PBMC结合，并在体外抑制多种有丝分裂原（Con A、PHA、PWM）诱导的增殖反应。还发现，尽管GM1与淋巴细胞的结合非常迅速（10 - 30分钟），但需要超过18小时的预孵育才能诱导淋巴细胞无反应性。GM1结合诱导淋巴细胞无反应状态可能需要一些细胞内事件。此外，癌症患者中CT结合淋巴细胞的数量以及每个细胞结合的CT量均显示增加。这些结果表明，血清和淋巴细胞表面GM1的增加可能是各种病理状态尤其是癌症患者中淋巴细胞反应性受抑制的机制之一。
