RajBhandary U L, Browning K S, Burke J, Yin S, Chang D
Princess Takamatsu Symp. 1982;12:41-51.
Our studies on Neurospora crassa mitochondria have included sequence analysis of tRNAs, mapping and cloning of the tRNA, rRNA, and protein genes and the DNA sequence analysis of these genes. Results from tRNA sequence analyses explain how the mitochondrial protein synthesizing system can function with a much smaller number of tRNAs than other systems. Mapping studies have shown that the two rRNA genes and almost all of the tRNA genes are clustered onto a third of the mitochondrial genome. The two rRNA genes and all of the tRNA genes are coded for by the same DNA strand. DNA sequence analysis has provided several interesting results. Twenty-four tRNA genes have been identified. Highly conserved GC rich palindromic sequences flank tRNA genes. The intervening sequence within the large rRNA gene has a long open reading frame capable of coding for a protein 426 amino acids long. The gene for cytochrome oxidase subunit 3 has been localized within the tRNA-rRNA gene cluster and has been sequenced. This gene is also flanked by the highly conserved GC rich palindromic DNA sequences.
我们对粗糙脉孢菌线粒体的研究包括tRNA的序列分析、tRNA、rRNA和蛋白质基因的定位与克隆以及这些基因的DNA序列分析。tRNA序列分析结果解释了线粒体蛋白质合成系统如何能够以比其他系统少得多的tRNA发挥功能。定位研究表明，两个rRNA基因和几乎所有的tRNA基因都聚集在线粒体基因组的三分之一区域。两个rRNA基因和所有的tRNA基因都由同一条DNA链编码。DNA序列分析得出了几个有趣的结果。已鉴定出24个tRNA基因。tRNA基因两侧是高度保守的富含GC的回文序列。大rRNA基因内的间隔序列有一个长的开放阅读框，能够编码一个426个氨基酸长的蛋白质。细胞色素氧化酶亚基3的基因已定位在tRNA-rRNA基因簇内并已测序。该基因两侧也有高度保守的富含GC的回文DNA序列。
