McKnight S L, Chao M, Sweet R W, Silverstein S, Axel R
Princess Takamatsu Symp. 1982;12:73-85.
Transformation, or DNA-mediated gene transfer, permits the stable introduction of new genetic information into a cell and therefore provides an opportunity to examine the expression of exogenous DNA sequences in the transformed host. A series of deletion mutants to analyze the sequences required for accurate and quantitative in vivo transcription of the herpes simplex virus (HSV) thymidine kinase (tk) gene has been constructed. Control of the efficiency of transcription as well as rough specification of the sites of initiation reside within sequences 40-110 nucleotides from the tk structural gene. The sequences responsible for induction of tk mRNA overlap this transcriptional control region. Analysis of the conformation of the integrated tk gene in the chromosome reveals that these DNA sequences are exquisitely sensitive to nuclease attack. The major heat shock gene of Drosophila and the human beta globin gene have also been introduced into murine cells to identify sequences responsible for induction of these finely regulated genes.
转化，即DNA介导的基因转移，可使新的遗传信息稳定导入细胞，因此为研究外源DNA序列在转化宿主中的表达提供了机会。已构建了一系列缺失突变体，用于分析单纯疱疹病毒（HSV）胸苷激酶（tk）基因在体内进行准确和定量转录所需的序列。转录效率的控制以及起始位点的大致定位位于距tk结构基因40 - 110个核苷酸的序列内。负责诱导tk mRNA的序列与该转录控制区域重叠。对染色体中整合的tk基因构象的分析表明，这些DNA序列对核酸酶攻击极为敏感。果蝇的主要热休克基因和人类β珠蛋白基因也已被导入鼠细胞，以鉴定负责诱导这些精细调控基因的序列。
