大鼠肋骨硫酸软骨素的异质性及对大鼠胃软骨硫酸酯酶的敏感性
Heterogeneity of rat rib chondroitin sulfate and susceptibility to rat gastric chondrosulfatase.
作者信息
Liau Y H, Galicki N I, Horowitz M I
出版信息
Biochim Biophys Acta. 1978 Mar 20;539(3):315-23. doi: 10.1016/0304-4165(78)90036-3.
Cartilage chondroitin sulfate isolated directly from rat rib or from in vitro culture of rat rib constitutes a population of glycosaminoglycans which is heterogeneous with respect to size, degree of sulfation and content of N-acetyl-galactosamine 4-sulfate. Fractions, elute from Dowex-1 in order of increasing molecular size and degree of sulfation up to a certain limit. Unsulfated disaccharides and disulfated disaccharides are present in both the undersulfated chondroitin sulfate fractions and in the average or more representative chondroitin sulfate. A small content of disccharide 6-sulfate is present in all fractions and appears to be an integral part of the chondroitin 4-sulfate molecules. Rat gastric chondrosulfatase hydrolyzes sulfate preferentially from the larger chondroitin 4-sulfate molecules, and the sulfate is removed primarily from the disaccharide 4-sulfate units.
直接从大鼠肋骨或大鼠肋骨体外培养物中分离得到的软骨硫酸软骨素构成了一组糖胺聚糖，它们在大小、硫酸化程度和4-硫酸-N-乙酰半乳糖胺含量方面具有异质性。各组分按照分子大小和硫酸化程度增加的顺序从Dowex -1上洗脱，直至达到一定限度。未硫酸化的二糖和二硫酸化的二糖同时存在于硫酸化不足的硫酸软骨素组分以及平均或更具代表性的硫酸软骨素中。所有组分中都含有少量的6-硫酸二糖，它似乎是4-硫酸软骨素分子的一个组成部分。大鼠胃软骨硫酸酯酶优先从较大的4-硫酸软骨素分子中水解硫酸，且硫酸主要从4-硫酸二糖单元中去除。
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