Wu C F, Berneking J M, Barker D L
J Neurochem. 1983 May;40(5):1386-96. doi: 10.1111/j.1471-4159.1983.tb13581.x.
A radiochemical method is applied to the study of neurotransmitter metabolism in Drosophila. The larval CNS is a favorable system for analyzing acetylcholine (ACh) metabolism, since the pool of [3H]ACh rapidly reaches a steady state with a high ratio of intracellular [3H]ACh to [3H]choline. A temperature-sensitive paralytic mutant, shibirets, shows reduced [3H]ACh accumulation at the restrictive temperature. This reduction is not the result of decreased synthesis of [3H]ACh, but rather an abnormally rapid rate of release, which is not prevented by blocking tetrodotoxin-sensitive nerve activity.
一种放射化学方法被应用于研究果蝇中的神经递质代谢。幼虫中枢神经系统是分析乙酰胆碱（ACh）代谢的有利系统，因为[3H]ACh库能迅速达到稳定状态，细胞内[3H]ACh与[3H]胆碱的比例很高。一种温度敏感的麻痹突变体，即shibirets，在限制温度下显示出[3H]ACh积累减少。这种减少不是[3H]ACh合成减少的结果，而是释放速率异常加快，而阻断河豚毒素敏感的神经活动并不能阻止这种情况。
