Prieto-Soto A, Gourlie B, Miwa M, Pigiet V, Sugimura T, Malik N, Smulson M
J Virol. 1983 Feb;45(2):600-6. doi: 10.1128/JVI.45.2.600-606.1983.
The host nuclear enzyme poly(ADP-ribose) polymerase has been shown to be associated with the replicative intermediate and mature forms of polyoma virus minichromosomes. Minichromosome-associated histones H2A and H2B as well as several nonhistone proteins were poly ADP-ribosylated by endogenous poly(ADP-ribose) polymerase. In addition, minichromosome fractions catalyzed the formation in vitro of dimers of endogenous histone H1 linked by poly(ADP-ribose). Poly ADP-ribosylated polyoma virus minichromosome chromatin labeled in vivo with [3H]thymidine could be retained and eluted from anti-poly(ADP-ribose) immunoglobulin G-Sepharose. Pulse-labeled replicative intermediate minichromosomes were retained better on the antibody columns than were mature minichromosomes labeled for 2.5 h. The possible role of poly ADP-ribosylation of viral nucleosomes during polyoma replication or transcription is discussed.
宿主核酶聚（ADP-核糖）聚合酶已被证明与多瘤病毒微型染色体的复制中间体和成熟形式相关。与微型染色体相关的组蛋白H2A和H2B以及几种非组蛋白都被内源性聚（ADP-核糖）聚合酶进行了聚ADP-核糖基化修饰。此外，微型染色体组分在体外催化了由聚（ADP-核糖）连接的内源性组蛋白H1二聚体的形成。用[3H]胸苷在体内标记的聚ADP-核糖基化多瘤病毒微型染色体染色质可以被保留，并从抗聚（ADP-核糖）免疫球蛋白G-琼脂糖凝胶上洗脱下来。脉冲标记的复制中间体微型染色体在抗体柱上的保留效果比标记2.5小时的成熟微型染色体更好。本文讨论了病毒核小体的聚ADP-核糖基化在多瘤病毒复制或转录过程中的可能作用。
