Malik N, Smulson M
Biochemistry. 1984 Jul 31;23(16):3721-5. doi: 10.1021/bi00311a023.
The chromatin-associated enzyme poly(ADP-Rib) polymerase catalyzes the posttranslational modifications of histones. Antibody to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] has been coupled to Sepharose, and the resulting immunoadsorbant was used to fractionate, specifically, oligonucleosomes derived from cells pulse labeled for the acetylation modifications of chromatin by incubation with [3H]acetate followed by treatment with sodium butyrate. Generally, about 50% of the histone H3 and H4 mass becomes acetylated under these conditions. Pulse-labeled acetylated regions of chromatin were selectively retained by the anti-poly(ADP-Rib)-Sepharose column due to the presence of endogenous poly(ADP-Rib) components. The data suggest that certain histone molecules might be mutally poly(ADP-ribosylated) and acetylated, and this phenomenon was further explored at the protein level in the accompanying paper [Wong, M., & Smulson, M. (1984) Biochemistry (following paper in this issue)].
与染色质相关的酶聚（ADP - 核糖）聚合酶催化组蛋白的翻译后修饰。抗聚（腺苷二磷酸核糖）[聚（ADP - 核糖）]抗体已与琼脂糖凝胶偶联，所得免疫吸附剂用于特异性分离寡核小体，这些寡核小体来自经[3H]乙酸孵育进行染色质乙酰化修饰，随后用丁酸钠处理的脉冲标记细胞。一般来说，在这些条件下，约50%的组蛋白H3和H4质量会被乙酰化。由于内源性聚（ADP - 核糖）成分的存在，染色质的脉冲标记乙酰化区域被抗聚（ADP - 核糖）-琼脂糖凝胶柱选择性保留。数据表明某些组蛋白分子可能相互进行聚（ADP - 核糖基化）和乙酰化，并且在随附论文[Wong, M., & Smulson, M. (1984) Biochemistry（本期后续论文）]中在蛋白质水平上进一步探讨了这一现象。
