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牛白血病病毒两种共价闭合环状前病毒DNA分子的检测、纯化及特性分析

Detection, purification, and characterization of two species of covalently closed circular proviral DNA molecules of bovine leukemia virus.

作者信息

Kashmiri S V, Mehdi R, Ferrer J F

出版信息

J Virol. 1983 Mar;45(3):1172-6. doi: 10.1128/JVI.45.3.1172-1176.1983.

DOI:10.1128/JVI.45.3.1172-1176.1983
PMID:6300454
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256529/
Abstract

Cocultivation of uninfected and bovine leukemia virus-producing bat cells yielded, in addition to the unintegrated linear DNA duplex, DNA molecules that migrated as 4.4- and 4.8-kilobase-pair DNA fragments in gel electrophoresis. These DNA molecules were purified by acid-phenol extraction and cleaved with restriction endonucleases EcoRI, and HindIII, which have one recognition site each on the bovine leukemia virus proviral DNA. Such cleavage generated DNA molecules of approximately 10.0 and 9.4 kilobase pairs, thus indicating the existence of two species of covalently closed circular molecules of bovine leukemia virus proviral DNA.

摘要

未感染和产生牛白血病病毒的蝙蝠细胞共培养，除了未整合的线性DNA双链外，还产生了在凝胶电泳中迁移为4.4和4.8千碱基对DNA片段的DNA分子。这些DNA分子通过酸酚抽提纯化，并用限制性内切酶EcoRI和HindIII切割，它们在牛白血病病毒前病毒DNA上各有一个识别位点。这种切割产生了大约10.0和9.4千碱基对的DNA分子，从而表明存在两种共价闭合环状的牛白血病病毒前病毒DNA分子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/b714aca006b5/jvirol00150-0285-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/87928fbc796a/jvirol00150-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/aa7c4f87fddd/jvirol00150-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/d58e8f37d972/jvirol00150-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/b714aca006b5/jvirol00150-0285-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/87928fbc796a/jvirol00150-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/aa7c4f87fddd/jvirol00150-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/d58e8f37d972/jvirol00150-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d77c/256529/b714aca006b5/jvirol00150-0285-b.jpg

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引用本文的文献

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Molecular cloning of covalently closed circular DNA of bovine leukemia virus.牛白血病病毒共价闭合环状DNA的分子克隆
J Virol. 1984 Feb;49(2):583-7. doi: 10.1128/JVI.49.2.583-587.1984.
2
The structure of cloned 3'-terminal RNA region of bovine leukemia virus (BLV).牛白血病病毒（BLV）克隆的3'末端RNA区域的结构
Nucleic Acids Res. 1983 Sep 10;11(17):6079-87. doi: 10.1093/nar/11.17.6079.

本文引用的文献

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Leukemogenesis by bovine leukemia virus: proviral DNA integration and lack of RNA expression of viral long terminal repeat and 3' proximate cellular sequences.牛白血病病毒引发的白血病：前病毒DNA整合以及病毒长末端重复序列和3' 近端细胞序列的RNA表达缺失
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Long terminal repeats of endogenous mouse mammary tumour virus contain a long open reading frame which extends into adjacent sequences.内源性小鼠乳腺肿瘤病毒的长末端重复序列包含一个延伸至相邻序列的长开放阅读框。
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Efficient production of xenotropic murine leukemia virus unintegrated proviral DNA by cocultivation.
通过共培养高效生产嗜异性小鼠白血病病毒未整合的前病毒DNA
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Restriction endonuclease mapping of linear unintegrated proviral DNA of bovine leukemia virus.牛白血病病毒线性未整合前病毒DNA的限制性内切酶图谱分析
J Virol. 1981 Apr;38(1):27-33. doi: 10.1128/JVI.38.1.27-33.1981.
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Two-dimensional agarose gel electrophoresis "SeaPlaque" agarose dimension.二维琼脂糖凝胶电泳“SeaPlaque”琼脂糖维度。
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A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
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Selective extraction of polyoma DNA from infected mouse cell cultures.从受感染的小鼠细胞培养物中选择性提取多瘤病毒DNA。
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