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通过共培养高效生产嗜异性小鼠白血病病毒未整合的前病毒DNA

Efficient production of xenotropic murine leukemia virus unintegrated proviral DNA by cocultivation.

作者信息

Joseph D R

出版信息

J Virol. 1981 Jun;38(3):1095-8. doi: 10.1128/JVI.38.3.1095-1098.1981.

DOI:10.1128/JVI.38.3.1095-1098.1981
PMID:6264153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC171251/
Abstract

Cocultivation of virus-producing cells and homologous uninfected cells yielded greater than a 10-fold increase in linear and superhelical proviral DNAs as compared with previously published techniques.

摘要

与先前发表的技术相比，病毒产生细胞与同源未感染细胞的共培养使线性和超螺旋前病毒DNA增加了10倍以上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b0/171251/f8c500110b38/jvirol00006-0301-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b0/171251/005c87c1aebc/jvirol00006-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b0/171251/fe4b3302cccb/jvirol00006-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b0/171251/f8c500110b38/jvirol00006-0301-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b0/171251/005c87c1aebc/jvirol00006-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b0/171251/fe4b3302cccb/jvirol00006-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b0/171251/f8c500110b38/jvirol00006-0301-b.jpg

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Efficient production of xenotropic murine leukemia virus unintegrated proviral DNA by cocultivation.通过共培养高效生产嗜异性小鼠白血病病毒未整合的前病毒DNA
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Detection, purification, and characterization of two species of covalently closed circular proviral DNA molecules of bovine leukemia virus.

本文引用的文献

1
Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration.含有反向片段的克隆环状莫洛尼鼠白血病病毒DNA分子的结构：对逆转录病毒整合的影响
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3932-6. doi: 10.1073/pnas.77.7.3932.
2
Selective extraction of polyoma DNA from infected mouse cell cultures.从受感染的小鼠细胞培养物中选择性提取多瘤病毒DNA。
J Mol Biol. 1967 Jun 14;26(2):365-9. doi: 10.1016/0022-2836(67)90307-5.
3
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
牛白血病病毒两种共价闭合环状前病毒DNA分子的检测、纯化及特性分析
J Virol. 1983 Mar;45(3):1172-6. doi: 10.1128/JVI.45.3.1172-1176.1983.
4
Comparison of the restriction endonuclease maps of unintegrated proviral DNAs from four xenotropic murine leukemia viruses.四种嗜异性小鼠白血病病毒未整合前病毒DNA的限制性内切酶图谱比较
J Virol. 1981 Dec;40(3):963-70. doi: 10.1128/JVI.40.3.963-970.1981.
在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
4
Infectious viral DNA of murine leukemia virus.鼠白血病病毒的传染性病毒DNA。
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4910-3. doi: 10.1073/pnas.72.12.4910.
5
Restriction endonuclease cleavage of linear and closed circular murine leukemia viral DNAs: discovery of a smaller circular form.线性和闭环小鼠白血病病毒DNA的限制性内切酶切割：一种较小环状形式的发现。
Cell. 1979 Feb;16(2):323-32. doi: 10.1016/0092-8674(79)90009-6.
6
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.通过使用硫酸葡聚糖将大的DNA片段从琼脂糖凝胶高效转移至重氮苄氧基甲基纸并进行快速杂交。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7. doi: 10.1073/pnas.76.8.3683.
7
Cleavage map of linear mouse sarcoma virus DNA.线性小鼠肉瘤病毒DNA的切割图谱。
Proc Natl Acad Sci U S A. 1977 Jan;74(1):29-33. doi: 10.1073/pnas.74.1.29.
8
Serological characterization of B-tropic viruses of C57BL mice: possible origin by recombination of endogenous N-tropic and xenotropic viruses.C57BL小鼠B嗜性病毒的血清学特征：可能起源于内源性N嗜性病毒和异嗜性病毒的重组
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Characterization and mapping of RNase T1-resistant oligonucleotides derived from the genomes of Akv and MCF murine leukemia viruses.源自Akv和MCF鼠白血病病毒基因组的核糖核酸酶T1抗性寡核苷酸的表征与定位
Proc Natl Acad Sci U S A. 1978 Jan;75(1):495-9. doi: 10.1073/pnas.75.1.495.
10
Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants.MCF鼠白血病病毒是包膜（env）基因重组体的生化证据。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4676-80. doi: 10.1073/pnas.74.10.4676.