Abe S, Nemoto N, Sasaki M
Mutat Res. 1983 Apr;109(1):83-90. doi: 10.1016/0027-5107(83)90097-0.
2 human hepatoma cell lines (C-HC-4 and C-HC-20), in which aryl hydrocarbon hydroxylase activity was induced with benz[alpha]anthracene in vitro to about 140- and 64-fold of the respective basal levels, yielded an increased frequency of sister-chromatid exchanges (SCEs) when exposed to benzo[alpha]pyrene (BP), 7,12-dimethylbenz[alpha]anthracene and 3-methylcholanthrene in vitro. Analysis of the metabolism of BP by these cells by high-pressure liquid chromatography revealed that both cell lines produced various BP metabolites including the proximate form BP-7,8-dihydrodiol which has been reported to be the most potent inducer of SCEs among the metabolites of BP. In addition, aflatoxin B1 and cyclophosphamide also induced SCEs in these cell lines. The above findings suggest that these cells may be capable of metabolizing a range of indirect mutagens/carcinogens into DNA-active forms. These cells may therefore serve as a useful test system in vitro for the detection of genotoxic agents, without the use of an exogenous activating system.
2种人肝癌细胞系（C-HC-4和C-HC-20），其芳烃羟化酶活性在体外经苯并[a]蒽诱导后分别提高至各自基础水平的约140倍和64倍，当在体外暴露于苯并[a]芘（BP）、7,12-二甲基苯并[a]蒽和3-甲基胆蒽时，姐妹染色单体交换（SCE）频率增加。通过高压液相色谱分析这些细胞对BP的代谢发现，这两种细胞系均产生多种BP代谢产物，包括近致癌物形式的BP-7,8-二氢二醇，据报道该物质是BP代谢产物中最有效的SCE诱导剂。此外，黄曲霉毒素B1和环磷酰胺也可在这些细胞系中诱导SCE。上述发现表明，这些细胞可能能够将一系列间接诱变剂/致癌物代谢为具有DNA活性的形式。因此，这些细胞可作为一种有用的体外检测系统，用于检测遗传毒性剂，而无需使用外源性活化系统。
