质子可阻断孤立视网膜视杆细胞的暗电流。
Protons block the dark current of isolated retinal rods.
作者信息
Mueller P, Pugh E N
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1892-6. doi: 10.1073/pnas.80.7.1892.
Abstract
Membrane currents of isolated frog rods were recorded with the suction pipette technique and tested by perfusion techniques for their sensitivity to H+. The following facts have been established. (i) Increased [H+] suppresses the Na+ conductance of the outer segment rapidly and reversibly. (ii) H+ acts in the rod interior. (iii) The [H+] necessary to cause a 50% decrement in Na+ conductance is inversely related to the [Ca2+] over 5 orders of magnitude. (iv) The sensitivity to H+ and the sensitivity to light, as a function of [Ca2+], have the same slope. Thus, H+ act like light in effecting membrane current suppression but behave as if their effect is mediated through Ca2+. Based on these results and properties of rod disk membrane phosphodiesterase, we propose that protons produced in the light-activated hydrolysis of cGMP liberate Ca2+ from the disks by ion exchange.
摘要
采用吸移管技术记录分离的蛙视杆细胞的膜电流，并用灌注技术测试其对H⁺的敏感性。已确定以下事实：（i）[H⁺]升高会迅速且可逆地抑制外段的Na⁺电导。（ii）H⁺在视杆细胞内部起作用。（iii）使Na⁺电导降低50%所需的[H⁺]与[Ca²⁺]在超过5个数量级范围内呈反比关系。（iv）作为[Ca²⁺]的函数，对H⁺的敏感性和对光的敏感性具有相同的斜率。因此，H⁺在影响膜电流抑制方面的作用类似于光，但表现得好像其作用是通过Ca²⁺介导的。基于这些结果以及视杆盘膜磷酸二酯酶的特性，我们提出，在cGMP光激活水解过程中产生的质子通过离子交换从盘膜中释放出Ca²⁺。
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引用本文的文献
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Protons suppress the dark current of frog retinal rods.质子抑制青蛙视网膜视杆细胞的暗电流。
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Light-induced losses and dark recovery rates of guanosine 3',5'-cyclic monophosphate in rod outer segments of intact amphibian photoreceptors.完整两栖类光感受器视杆外段中3',5'-环磷酸鸟苷的光诱导损失及暗恢复率
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