Heitzman R J, Harwood D J
Vet Rec. 1983 Feb 5;112(6):120-3. doi: 10.1136/vr.112.6.120.
A fast, reliable and sensitive radioimmunoassay method using either 3H-hexoestrol or a 125I-hexoestrol derivative as the competitive radioligand has been developed for the measurement of residues of hexoestrol in faeces. The blank value for untreated cattle was 113 +/- 98 pg/g faeces, the intra-assay coefficient of variation 10.8 per cent, interassay coefficient of variation 11.1 per cent and recovery of 3H-hexoestrol added to faeces was 58.7 per cent. The concentration of hexoestrol in faeces was measured in 18 steers and 14 bulls at regular intervals after implantation of 36, 45 and 60 mg of hexoestrol. Residues were detected in all samples up to 104 days after implantation and in nine out of 13 samples taken 111 to 153 days after implantation. Residues of hexoestrol were also measured in edible tissues and body fluids of 14 bulls and eight steers slaughtered between 90 and 153 days after implantation of 45 mg hexoestrol. The percentages of samples containing residues were 82, 73, 64, 82 and 27 per cent for bile, urine, liver, kidney and muscle, respectively.
已开发出一种快速、可靠且灵敏的放射免疫分析方法，该方法使用³H-己烷雌酚或¹²⁵I-己烷雌酚衍生物作为竞争性放射性配体，用于测定粪便中己烷雌酚的残留量。未处理牛的空白值为113±98 pg/g粪便，批内变异系数为10.8%，批间变异系数为11.1%，添加到粪便中的³H-己烷雌酚回收率为58.7%。在植入36、45和60 mg己烷雌酚后，定期测定18头阉牛和14头公牛粪便中己烷雌酚的浓度。在植入后长达104天的所有样本中均检测到残留，在植入后111至153天采集的13个样本中有9个检测到残留。还测定了在植入45 mg己烷雌酚后90至153天之间屠宰的14头公牛和8头阉牛的可食用组织和体液中己烷雌酚的残留量。胆汁、尿液、肝脏、肾脏和肌肉中含有残留的样本百分比分别为82%、73%、64%、82%和27%。
