在用α/β干扰素处理的对干扰素耐药的弗瑞德白血病细胞变体中，尽管存在高亲和力的干扰素受体位点，但2-5A合成酶活性并未增加。
2-5A synthetase activity does not increase in interferon-resistant Friend leukemia cell variants treated with alpha/beta interferon despite the presence of high-affinity interferon receptor sites.
作者信息
Affabris E, Romeo G, Belardelli F, Jemma C, Mechti N, Gresser I, Rossi G B
出版信息
Virology. 1983 Mar;125(2):508-12. doi: 10.1016/0042-6822(83)90224-6.
The presence of interferon (IFN) receptors on mouse Friend leukemia cells (FLC) has been investigated in binding experiments with highly purified 125I-labeled mouse alpha/beta IFN. Both IFN-resistant clones and wild-type IFN-sensitive FLC showed a specific saturable binding site for mouse IFN with a similar affinity constant. In contrast to IFN-sensitive FLC, IFN-resistant FLC variants were not inducible by IFN for double-stranded RNA-dependent 2-5A synthetase activity.
通过使用高度纯化的125I标记的小鼠α/β干扰素进行结合实验，对小鼠Friend白血病细胞（FLC）上干扰素（IFN）受体的存在情况进行了研究。干扰素抗性克隆和野生型干扰素敏感FLC均显示出对小鼠干扰素具有特异性的可饱和结合位点，且亲和力常数相似。与干扰素敏感的FLC不同，干扰素抗性FLC变体不能被干扰素诱导产生双链RNA依赖性2-5A合成酶活性。
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