Gresser I, Moss J, Woodrow D, Le Bousse C, Maury C, Proietti E, Belardelli F
Institute de Recherches Scientifiques sur le Cancer, Villejuif, France.
Am J Pathol. 1991 May;138(5):1125-33.
Friend erythroleukemia cells (FLC) passaged in mice are highly tumorigenic and multiply extensively in the livers of suckling DBA/2 mice without differentiating. In contrast, in vitro passaged FLCs injected intravenously were of low tumorigenicity, multiplied to a limited extent in the livers of suckling mice, and underwent marked differentiation from the proerythroblast to the orthochromatic erythroblast stage in the liver. The presence of characteristic C-type virions budding from the cell surface in various stages of erythroid differentiation served as a marker of the injected FLCs. When the same in vitro passaged FLCs that differentiated in the liver were injected subcutaneously in suckling mice, they formed large subcutaneous tumors consisting of sheets of undifferentiated tumor cells. It is concluded that the tumorigenicity of FLCs depended on the site of tumor growth and that there is an inverse correlation between the tumorigenic capacity and the capacity to differentiate.
在小鼠体内传代的Friend红白血病细胞（FLC）具有高度致瘤性，可在新生DBA/2小鼠的肝脏中大量增殖且不发生分化。相比之下，静脉注射体外传代的FLC致瘤性较低，在新生小鼠肝脏中增殖程度有限，并在肝脏中从早幼红细胞阶段向正成红细胞阶段发生显著分化。在红系分化的各个阶段，细胞表面出现特征性的C型病毒颗粒芽生，作为注入的FLC的标志物。当将在肝脏中发生分化的相同体外传代FLC皮下注射到新生小鼠体内时，它们形成了由成片未分化肿瘤细胞组成的大型皮下肿瘤。得出的结论是，FLC的致瘤性取决于肿瘤生长部位，并且致瘤能力与分化能力之间存在负相关。
