Kazakevich T B, Gervazieva V B, Bazhanova I G, Kanchurin A Kh
Zh Mikrobiol Epidemiol Immunobiol. 1983 Jan(1):103-7.
B. pertussis protein fraction obtained by precipitation with trichloroacetic acid (TCA) stimulated the development of clinically and histologically pronounced experimental allergic encephalomyelitis (EAE) in guinea pigs when introduced together with a heterologous cerebral antigen, the two compounds forming an encephalitogenic mixture. The adjuvant activity of the TCA-precipitated fraction depended on its dose. The sera of the animals with EAE induced by the encephalitogenic mixture containing B. pertussis cells or TCA-precipitated fraction showed a cytopathogenic effect in the monolayer culture of newborn rat cerebellum cells. The cytopathogenic effect was more pronounced in the sera obtained at the period of the development of the clinical symptoms of the disease (days 14-18), while the cytotoxic effect of the sera obtained on day 30 after immunization decreased irrespective of the manifestations of EAE, this decrease being in correlation with the dose of the TCA-precipitated fraction in the encephalitogenic mixture.
用三氯乙酸（TCA）沉淀获得的百日咳杆菌蛋白组分，与一种异源性脑抗原一起引入时，能刺激豚鼠发生临床和组织学上明显的实验性变应性脑脊髓炎（EAE），这两种化合物形成一种致脑炎混合物。TCA沉淀组分的佐剂活性取决于其剂量。由含有百日咳杆菌细胞或TCA沉淀组分的致脑炎混合物诱导发生EAE的动物血清，在新生大鼠小脑细胞单层培养中显示出细胞致病作用。在疾病临床症状出现期（第14 - 18天）获得的血清中，细胞致病作用更为明显，而免疫后第30天获得的血清的细胞毒性作用则降低，无论EAE的表现如何，这种降低与致脑炎混合物中TCA沉淀组分的剂量相关。
