Bernal J, Saukkonen-Bernal S
Acta Endocrinol Suppl (Copenh). 1983;251:16-20.
A method of T3 binding to human mononuclear cells isolated from buffy coat samples is described. Freshly isolated mononuclear cells bind T3 with Ka = 8.6 +/- 1.2 M-1 and Bmax = 6.5 +/- 1.5 fmol/100 micrograms DNA. The preparations of monoclear cells contained up to 25% monocytes, and the binding of T3 to lymphocytes and monocytes was compared after removing the monocytes. On the average, Bmax was slightly higher in monocytes (10.1 +/- 5.8) than in lymphocytes (7.0 +/- 3.2) but the difference was not statistically significant. In some experiments, however, Bmax was clearly higher in monocytes than in lymphocytes. After culturing lymphocytes in Newborn Calf Serum containing medium, Ka decreased and Bmax increased. The T3 binding method described may be used to study the effect of regulatory factors on the T3 receptor in human lymphocytes in culture.
本文描述了一种从血沉棕黄层样本中分离出的人单核细胞与T3结合的方法。新鲜分离的单核细胞结合T3的解离常数（Ka）为8.6±1.2 M-1，最大结合容量（Bmax）为6.5±1.5 fmol/100微克DNA。单核细胞制剂中含有高达25%的单核细胞，去除单核细胞后比较了T3与淋巴细胞和单核细胞的结合情况。平均而言，单核细胞的Bmax（10.1±5.8）略高于淋巴细胞（7.0±3.2），但差异无统计学意义。然而，在一些实验中，单核细胞的Bmax明显高于淋巴细胞。在含新生小牛血清的培养基中培养淋巴细胞后，Ka降低，Bmax增加。所描述的T3结合方法可用于研究调节因子对培养的人淋巴细胞中T3受体的影响。
