Carlson C A
Antonie Van Leeuwenhoek. 1982;48(6):555-67. doi: 10.1007/BF00399541.
The genetics of denitrification is a relatively unexplored area that has great promise. Species of Pseudomonas are probably best suited for study because they are widely found among natural denitrifying populations and are quite readily amenable to genetic analysis. The techniques for mutagenesis and for the exchange of chromosomal genes to characterize mutant strains have been well-developed in P. aeruginosa and are being developed in P. stutzeri. Mutants defective in the denitrification of nitrate, nitrite, and nitrous oxide are now available and will aid in describing the catalytic and regulatory elements of the denitrification pathway.
反硝化作用的遗传学是一个相对未被探索但前景广阔的领域。假单胞菌属的物种可能最适合用于研究，因为它们在天然反硝化菌群中广泛存在，并且很容易进行遗传分析。在铜绿假单胞菌中，诱变和染色体基因交换以鉴定突变菌株的技术已经很成熟，在施氏假单胞菌中也正在开发这些技术。目前已有在硝酸盐、亚硝酸盐和一氧化二氮反硝化方面存在缺陷的突变体，这将有助于描述反硝化途径的催化和调控元件。
