维拉帕米可提高DNA介导的基因转移在哺乳动物细胞中的效率。

Verapamil enhances the efficiency of DNA-mediated gene transfer in mammalian cells.

作者信息

Akiyama S, Ono M, Kuwano M

出版信息

Biochem Biophys Res Commun. 1983 Feb 10;110(3):783-8. doi: 10.1016/0006-291x(83)91030-6.

DOI:10.1016/0006-291x(83)91030-6

Abstract

Treatment of Ltk- cells with the calcium antagonists, verapamil and diltiazem, but not nifedipine, causes a 3-fold enhancement of the frequency of transfer of the cloned gene for herpes simplex virus thymidine kinase (HSV-tk). The frequency of phenotypic expression of the HSV-tk DNA was 20 to 34 times higher than that of genotypic transformation. Phenotypic expression was also 2.3 to 2.6 times increased when 20 micrograms/ml of verapamil was present during calcium phosphate-mediated DNA transfection.

摘要

用钙拮抗剂维拉帕米和地尔硫䓬（而非硝苯地平）处理Ltk-细胞，可使单纯疱疹病毒胸苷激酶（HSV-tk）克隆基因的转移频率提高3倍。HSV-tk DNA的表型表达频率比基因型转化频率高20至34倍。在磷酸钙介导的DNA转染过程中，当存在20微克/毫升维拉帕米时，表型表达也增加了2.3至2.6倍。

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