由人肝母细胞瘤建立的HUH6 C15细胞系产生纤连蛋白。

Production of fibronectin by HUH6 C15 cell line established from a human hepatoblastoma.

作者信息

Tanaka M, Kawamura K, Fang M, Higashino K, Kishimoto S, Nakabayashi H, Sato J

出版信息

Biochem Biophys Res Commun. 1983 Feb 10;110(3):837-41. doi: 10.1016/0006-291x(83)91037-9.

Abstract

Fibronectin was detected by indirect immunofluorescence on the cell surfaces of HUH6 C15 cells, established from a human hepatoblastoma and maintained with serum-free RPMI 1640 medium. Fibronectin synthesized by HUH6 Cl5 was purified by gelatin-Sepharose affinity chromatography and compared with human plasma fibronectin in respect to molecular weight, electrophoretic mobility and antigenicity. Fibronectin synthesized by this cell line was proved to be identical with human plasma fibronectin.

摘要

通过间接免疫荧光法在人肝母细胞瘤来源的HUH6 C15细胞表面检测到纤连蛋白，该细胞用无血清RPMI 1640培养基培养。通过明胶-琼脂糖亲和层析法纯化HUH6 Cl5合成的纤连蛋白，并在分子量、电泳迁移率和抗原性方面与人血浆纤连蛋白进行比较。结果证明该细胞系合成的纤连蛋白与人血浆纤连蛋白相同。