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通过辐射失活法测定尼群地平敏感型Ca2+通道的分子大小

Determination of the molecular size of the nitrendipine-sensitive Ca2+ channel by radiation inactivation.

作者信息

Norman R I, Borsotto M, Fosset M, Lazdunski M, Ellory J C

出版信息

Biochem Biophys Res Commun. 1983 Mar 29;111(3):878-83. doi: 10.1016/0006-291x(83)91381-5.

DOI:10.1016/0006-291x(83)91381-5
PMID:6301488
Abstract

The molecular size of the [3H] nitrendipine receptor of transverse tubules prepared from rabbit skeletal muscle and from rat cortex synaptic membranes have been investigated. Radiation inactivation of the specific binding of [3H] nitrendipine was consistent with Mr equals 210 000 +/- 20,000 for the receptor in each membrane preparation indicating a common size for the [3H] nitrendipine receptor.

摘要

对从兔骨骼肌制备的横管以及大鼠皮质突触膜中的[3H]尼群地平受体的分子大小进行了研究。[3H]尼群地平特异性结合的辐射失活表明，每种膜制剂中的受体的分子量为210 000±20 000，这表明[3H]尼群地平受体具有共同的大小。

相似文献

1
Determination of the molecular size of the nitrendipine-sensitive Ca2+ channel by radiation inactivation.通过辐射失活法测定尼群地平敏感型Ca2+通道的分子大小
Biochem Biophys Res Commun. 1983 Mar 29;111(3):878-83. doi: 10.1016/0006-291x(83)91381-5.
2
[3H]nitrendipine labelling of the Ca2+ channel in skeletal muscle.
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[3H]nitrendipine-labeled calcium channels discriminate inorganic calcium agonists and antagonists.[3H]尼群地平标记的钙通道可区分无机钙激动剂和拮抗剂。
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Binding of [3H]nitrendipine to heart and brain membranes from normotensive and spontaneously hypertensive rats.[3H]尼群地平与正常血压及自发性高血压大鼠心脏和脑膜的结合。
Eur J Pharmacol. 1983 Mar 25;88(2-3):277-8. doi: 10.1016/0014-2999(83)90020-1.

引用本文的文献

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Unitary behavior of skeletal, cardiac, and chimeric L-type Ca2+ channels expressed in dysgenic myotubes.在发育不全的肌管中表达的骨骼肌、心肌和嵌合L型Ca2+通道的单一性行为。
J Gen Physiol. 1996 Jun;107(6):731-42. doi: 10.1085/jgp.107.6.731.
2
Functional interactions of calcium-antagonists in K+-depolarized smooth muscle.钙拮抗剂在钾离子去极化平滑肌中的功能相互作用。
Br J Pharmacol. 1983 Nov;80(3):485-8. doi: 10.1111/j.1476-5381.1983.tb10719.x.
3
Calcium channels: evidence for oligomeric nature by target size analysis.钙通道：通过靶点大小分析证明其寡聚性质
EMBO J. 1983;2(10):1729-32. doi: 10.1002/j.1460-2075.1983.tb01649.x.
4
Calcium channels: molecular pharmacology, structure and regulation.钙通道：分子药理学、结构与调控
J Membr Biol. 1988 Sep;104(2):81-105. doi: 10.1007/BF01870922.
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Photoaffinity-labelling of the calcium-channel-associated 1,4-dihydropyridine and phenylalkylamine receptor in guinea-pig hippocampus. A 195 kDa polypeptide carries both drug receptors and has similarities to the alpha 1 subunit of the purified skeletal-muscle calcium channel.豚鼠海马体中钙通道相关的1,4 - 二氢吡啶和苯烷基胺受体的光亲和标记。一种195 kDa的多肽同时携带这两种药物受体，并且与纯化的骨骼肌钙通道的α1亚基具有相似性。
Biochem J. 1988 Jul 1;253(1):39-47. doi: 10.1042/bj2530039.
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Heterogeneity of calcium channels from a purified dihydropyridine receptor preparation.来自纯化的二氢吡啶受体制剂的钙通道异质性。
Biophys J. 1987 Nov;52(5):891-9. doi: 10.1016/S0006-3495(87)83283-6.
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Photoaffinity labelling of the cardiac calcium channel. (-)-[3H]azidopine labels a 165 kDa polypeptide, and evidence against a [3H]-1,4-dihydropyridine-isothiocyanate being a calcium-channel-specific affinity ligand.心脏钙通道的光亲和标记。（-）-[³H]叠氮平标记一种165 kDa的多肽，以及关于[³H]-1,4-二氢吡啶-异硫氰酸盐不是钙通道特异性亲和配体的证据。
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The molecular pharmacology and structural features of calcium channels.钙通道的分子药理学及结构特征
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