Estival A, Mouniélou P, Trocheris V, Scemama J L, Clemente F, Hollande E, Ribet A
Biochem Biophys Res Commun. 1983 Mar 29;111(3):958-63. doi: 10.1016/0006-291x(83)91393-1.
It is known that the human exocrine pancreas responds to secretin stimulation more than does VIP, a structurally related peptide. We looked for the receptors for those polypeptides in a human pancreatic cancer cell line grown in culture and in nude mice. By analysing the cAMP responses and the 125I-VIP binding we found VIP receptors with a KD of 1.5 10(-9) M. Secretin stimulates the adenylate cyclase through the VIP receptor sites with a KD of 1.7. 10(-6) M. We noted also that during cell proliferation in culture there was about a 5 fold increase of the cAMP response to VIP.
已知人类外分泌胰腺对促胰液素刺激的反应比对结构相关肽血管活性肠肽（VIP）的反应更强。我们在培养的人胰腺癌细胞系以及裸鼠中寻找这些多肽的受体。通过分析环磷酸腺苷（cAMP）反应和¹²⁵I-VIP结合情况，我们发现VIP受体的解离常数（KD）为1.5×10⁻⁹ M。促胰液素通过KD为1.7×10⁻⁶ M的VIP受体位点刺激腺苷酸环化酶。我们还注意到，在细胞培养增殖过程中，对VIP的cAMP反应增加了约5倍。
