血管活性肠肽：培养的肠道癌细胞系中3':5'-环磷酸腺苷积累的强效刺激物。
Vasoactive intestinal peptide: a potent stimulator of adenosine 3':5'-cyclic monophosphate accumulation in gut carcinoma cell lines in culture.
作者信息
Laburthe M, Rousset M, Boissard C, Chevalier G, Zweibaum A, Rosselin G
出版信息
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2772-5. doi: 10.1073/pnas.75.6.2772.
Vasoactive intestinal peptide (VIP) is a potent and efficient stimulator of adenosine 3':5'-cyclic monophosphate (cAMP) accumulation in a human colon carcinoma cell line, HT 29. cAMP accumulation is sensitive to a concentration of VIP as low as 3x10(-12) M. Maximum VIP-induced cAMP levels were observed with 10(-9) M VIP and are about 200 times above the basal levels. Half-maximum cAMP production was obtained at 3x10(-10) M VIP. (125)I-Labeled VIP was found to bind to HT 29 cells; this binding was competitively inhibited by concentrations of unlabeled VIP between 10(-10) and 10(-7) M. Half-maximum inhibition of binding was observed with 2x10(-9) M VIP. Secretin also stimulated cAMP accumulation in HT 29 cells, but its effectiveness was 1/1000 that of VIP. The other peptides tested at 10(-7) M, such as insulin, glucagon, bovine pancreatic polypeptide, somatostatin, octapeptide of cholecystokinin, neurotensin, and substance P, did not stimulate cAMP accumulation. Prostaglandin E(1) and catecholamines stimulated cAMP production but were 1/2.3 and 1/5.5 as efficient as VIP, respectively. Another malignant cell line from the gut, the human rectal tumor cell line HRT 18, is also sensitive to VIP. In HRT 18 cells, VIP stimulated cAMP accumulation with a maximal effect at 10(-8) M; half-maximum stimulation was observed at about 10(-9) M. These results demonstrate the presence of VIP receptors in two malignant human intestinal cell lines (HT 29 and HRT 18) in culture and provide a model for studying the action of VIP on cell proliferation.
血管活性肠肽（VIP）是一种强效且高效的刺激物，可刺激人结肠癌细胞系HT 29中3':5'-环磷酸腺苷（cAMP）的积累。cAMP积累对低至3×10⁻¹² M的VIP浓度敏感。用10⁻⁹ M VIP观察到最大VIP诱导的cAMP水平，比基础水平高出约200倍。在3×10⁻¹⁰ M VIP时获得半最大cAMP产量。发现¹²⁵I标记的VIP与HT 29细胞结合；这种结合被10⁻¹⁰至10⁻⁷ M浓度的未标记VIP竞争性抑制。用2×10⁻⁹ M VIP观察到结合的半最大抑制。促胰液素也刺激HT 29细胞中cAMP的积累，但其效力仅为VIP的1/1000。在10⁻⁷ M测试的其他肽，如胰岛素、胰高血糖素、牛胰多肽、生长抑素、胆囊收缩素八肽、神经降压素和P物质，均未刺激cAMP积累。前列腺素E₁和儿茶酚胺刺激cAMP产生，但效率分别仅为VIP的1/2.3和1/5.5。另一种来自肠道的恶性细胞系，人直肠肿瘤细胞系HRT 18，也对VIP敏感。在HRT 18细胞中，VIP刺激cAMP积累，在10⁻⁸ M时具有最大效应；在约10⁻⁹ M时观察到半最大刺激。这些结果证明了在培养的两种人恶性肠道细胞系（HT 29和HRT 18）中存在VIP受体，并为研究VIP对细胞增殖的作用提供了一个模型。
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