Fitzgerald J W, Franklin B L
Can J Microbiol. 1982 Dec;28(12):1296-9. doi: 10.1139/m82-193.
The ability of primary alkylsulfate esters and alkanesulfonates to induce alkylsulfatase formation in Pseudomonas aeruginosa was compared on the basis of maximum enzyme levels, induction rate, and levels of induction as a function of inducer concentration. Apparent K inducer values for these effectors were calculated from linear relationships between reciprocals of induction rate and inducer concentration. Maximum enzyme levels estimated from linear progress relationships for each effector indicated that little major distinction could be made between effectors. Excepting carbon chain lengths of C8 which induced about the same level of enzyme, sulfate esters were generally better inducers than sulfonates with little or no apparent induction occurring with effectors of chain length less than or equal to C6. These observations also held true when rates were compared, except that the rate for the C8 ester was approximately ninefold greater than that for the analogous sulfonate. Apparent K inducer constants decreased with increasing alkyl chain length for the esters (C6-C12) and the sulfonates (C8-C14). Values for the esters were approximately sixfold greater than those for sulfonates of equivalent chain length. Plots of log apparent K inducer values against carbon chain length for each series of esters and sulfonates yielded straight-line relationships characteristic of an homologous series in each instance.
基于最大酶水平、诱导速率以及诱导水平与诱导剂浓度的函数关系，对伯烷基硫酸酯和链烷磺酸盐在铜绿假单胞菌中诱导烷基硫酸酯酶形成的能力进行了比较。根据诱导速率的倒数与诱导剂浓度之间的线性关系，计算了这些效应物的表观K诱导剂值。根据每种效应物的线性进程关系估算的最大酶水平表明，效应物之间几乎没有明显差异。除了碳链长度为C8的诱导剂诱导的酶水平大致相同外，硫酸酯通常比磺酸盐是更好的诱导剂，碳链长度小于或等于C6的效应物几乎没有或没有明显的诱导作用。当比较速率时，这些观察结果同样成立，只是C8酯的速率比类似磺酸盐的速率大约高九倍。对于酯（C6 - C12）和磺酸盐（C8 - C14），表观K诱导剂常数随着烷基链长度的增加而降低。酯的值大约是等效链长度磺酸盐值的六倍。针对每个系列的酯和磺酸盐，将表观K诱导剂值的对数与碳链长度作图，在每种情况下都得到了同系物系列特有的直线关系。
