Kemp C W, Curtis M A, Robrish S A, Bowen W H
FEBS Lett. 1983 May 2;155(1):61-4. doi: 10.1016/0014-5793(83)80209-9.
Dental plaque samples collected from monkeys (Macaca mulatta) were found to contain a large amount of dissolved methane gas (0.6 nmol CH4/mg wet wt plaque). Enrichment cultures inoculated with dental plaque obtained from Macaca fascicularis produced methane when the medium contained ethanol, methanol, lactate, acetate or a hydrogen + CO2 atmosphere. Methane formation in the enrichments was inhibited by oxidation of the culture medium, autoclaving or the addition of 2-bromoethane sulfonic acid (BES). The methane producing enrichments were observed to contain fluorescent cocci occurring singly and in short chains. It was concluded that methane formation in the monkey dental plaque was the result of the presence of methanogenic bacteria.
从恒河猴（猕猴）采集的牙菌斑样本中发现含有大量溶解的甲烷气体（0.6纳摩尔CH₄/毫克湿重牙菌斑）。用食蟹猴的牙菌斑接种的富集培养物，当培养基含有乙醇、甲醇、乳酸盐、乙酸盐或氢气+二氧化碳气氛时会产生甲烷。富集培养物中的甲烷形成受到培养基氧化、高压灭菌或添加2-溴乙烷磺酸（BES）的抑制。观察到产生甲烷的富集培养物中含有单个和短链形式的荧光球菌。得出的结论是，猴牙菌斑中的甲烷形成是产甲烷细菌存在的结果。
