Belay N, Johnson R, Rajagopal B S, Conway de Macario E, Daniels L
Department of Microbiology, University of Iowa, Iowa City 52242.
Appl Environ Microbiol. 1988 Feb;54(2):600-3. doi: 10.1128/aem.54.2.600-603.1988.
Samples of human dental plaque were examined for the presence of methanogenic bacteria. Of 54 samples from 36 patients, 20 yielded H2/CO2-using methanogenic enrichment cultures. All methanogen-positive samples were from patients with some degree of periodontal disease. The predominant populations in the enrichments had morphologies characteristic of Methanobrevibacter spp. In six enrichments derived from three patients, the common methanogen was antigenically similar to Methanobrevibacter smithii. The same was true for the three methanogenic isolates obtained in axenic culture from a fourth patient. The six enrichments and two of the three isolates were antigenically closer to strain ALI than to PS. Two of the enrichments also had subpopulations with weak antigenic similarity to Methanosphaera stadtmanae. The data indicate that methanogens in the oral cavity of humans are antigenically close to those found in the intestinal tract.
对人类牙菌斑样本进行了产甲烷菌检测。在来自36名患者的54个样本中，20个样本产生了利用H2/CO2的产甲烷富集培养物。所有产甲烷菌阳性样本均来自患有某种程度牙周病的患者。富集培养物中的主要菌群具有短柄产甲烷杆菌属的形态特征。在来自三名患者的六个富集培养物中，常见的产甲烷菌在抗原性上与史氏短柄产甲烷杆菌相似。从第四名患者的无菌培养物中获得的三个产甲烷菌分离株也是如此。六个富集培养物以及三个分离株中的两个在抗原性上与ALI菌株比与PS菌株更接近。其中两个富集培养物还具有与史氏甲烷球形菌抗原性相似性较弱的亚群。数据表明，人类口腔中的产甲烷菌在抗原性上与肠道中的产甲烷菌相近。
