Lurie D P, DeLustro F, LeRoy E C
Inflammation. 1983 Mar;7(1):49-56. doi: 10.1007/BF00918007.
Effects of glucocorticoids on human neutrophil responses to leukocyte migration inhibition factor (LIF) and neutrophil chemokinesis were examined using an agarose gel technique. The roles of endogenous monohydroxyeicosatetraenoic acids (HETE) and prostaglandins (PG) in basal neutrophil chemokinesis were also examined. Methylprednisolone sodium succinate (MPSS) in concentrations up to 200 micrograms/ml failed to inhibit the neutrophil response to LIF. MPSS enhanced neutrophil chemokinesis in a dose-related manner at concentrations from 2 micrograms/ml to 200 micrograms/ml (P less than 0.01). Since inhibition of membrane phospholipase activity by MPSS is known to decrease production of HETE and PG, the present data suggest that HETE and PG do not mediate basal neutrophil chemokinesis. This was confirmed by selectively inhibiting HETE production with the lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA) or PG production with the cyclooxygenase inhibitor indomethacin. Neutrophil chemokinesis was unaffected by 10(-5) M NDGA (P less than 0.05) or 10(-5) indomethacin (P less than 0.05).
使用琼脂糖凝胶技术研究了糖皮质激素对人中性粒细胞对白细胞迁移抑制因子（LIF）反应及中性粒细胞趋化运动的影响。还研究了内源性单羟基二十碳四烯酸（HETE）和前列腺素（PG）在基础中性粒细胞趋化运动中的作用。浓度高达200微克/毫升的琥珀酸钠甲泼尼龙（MPSS）未能抑制中性粒细胞对LIF的反应。MPSS在2微克/毫升至200微克/毫升的浓度范围内以剂量相关方式增强中性粒细胞趋化运动（P<0.01）。由于已知MPSS抑制膜磷脂酶活性会减少HETE和PG的产生，目前的数据表明HETE和PG不介导基础中性粒细胞趋化运动。用脂氧合酶抑制剂去甲二氢愈创木酸（NDGA）选择性抑制HETE产生或用环氧化酶抑制剂吲哚美辛抑制PG产生证实了这一点。10^(-5)M NDGA（P<0.05）或10^(-5)吲哚美辛（P<0.05）对中性粒细胞趋化运动无影响。
