Roy A, Danchin A, Joseph E, Ullmann A
J Mol Biol. 1983 Mar 25;165(1):197-202. doi: 10.1016/s0022-2836(83)80251-4.
To study the regulation of Escherichia coli adenylate cyclase, plasmids that carry part of the cya gene, as well as plasmids containing hybrid genes having part of cya fused to part of lacZ, were constructed. This allowed us to propose that the enzyme is made of at least two well-defined domains. The NH2 terminus carries the ATP leads to cAMP catalytic activity, whereas the COOH-terminal end corresponds to a regulatory polypeptide domain.
为了研究大肠杆菌腺苷酸环化酶的调控机制，构建了携带部分cya基因的质粒，以及含有cya部分与lacZ部分融合的杂交基因的质粒。这使我们能够提出该酶至少由两个明确的结构域组成。NH2末端具有ATP生成cAMP的催化活性，而COOH末端对应于一个调节多肽结构域。
