Koop A H, Hartley M, Bourgeois S
Gene. 1984 May;28(2):133-46. doi: 10.1016/0378-1119(84)90250-6.
A 9500-bp DNA segment containing the adenylate cyclase gene (cya) of Escherichia coli has been isolated and analyzed. Four large proteins are encoded within this fragment - the adenylate cyclase protein (92 kDal), two proteins of unknown function (37 and 32 kDal), and a part of the uvrD-coded protein. Various truncated adenylate cyclase proteins, made from cya genes having as much as 60% of their carboxy-terminal end deleted, are sufficient to complement cya- hosts. When these truncated cya genes are present on a multicopy plasmid in a cya- host, the synthesis of beta-galactosidase is still regulated by glucose. The "maxicell" technique was used to visualize the four proteins encoded by this region and some of the truncated adenylate cyclase proteins.
一段包含大肠杆菌腺苷酸环化酶基因（cya）的9500碱基对DNA片段已被分离和分析。该片段编码四种大蛋白——腺苷酸环化酶蛋白（92千道尔顿）、两种功能未知的蛋白（37和32千道尔顿）以及uvrD编码蛋白的一部分。由cya基因产生的各种截短的腺苷酸环化酶蛋白，其羧基末端多达60%被删除，仍足以互补cya-宿主。当这些截短的cya基因存在于cya-宿主的多拷贝质粒上时，β-半乳糖苷酶的合成仍受葡萄糖调节。“大细胞”技术被用于观察该区域编码的四种蛋白以及一些截短的腺苷酸环化酶蛋白。
