Blostein R, Drapeau P, Benderoff S, Weigensberg A M
Can J Biochem Cell Biol. 1983 Jan;61(1):23-8. doi: 10.1139/o83-004.
Changes in the sodium pump and sodium-activated ATP hydrolysis (Na+-ATPase) were followed during maturation of low potassium (LK) and high potassium (HK) sheep reticulocytes released into circulation following massive bleeding. In vitro maturation of LK cell reticulocytes resulted in a progressive decrease in cellular K+, ouabain-sensitive 86Rb+ uptake, and Na+-ATPase activity. Although in vitro maturation (up to 10 days) did not result in changes in kinetic behaviour from that typical of reticulocytes to that typical of mature cells, kinetic changes were clearly evident after longer term maturation in vivo. This was shown for HK cells by comparing the characteristics of Na+-ATPase (response to K+) of the lightest (youngest) and heaviest (oldest) cell fractions of blood obtained at various periods during recovery following massive bleeding. It is concluded that the kinetic difference between immature and mature cells is due to cell maturation and reflects either a selective change and (or) loss of certain type(s) of pump sites.
在大量失血后释放到循环系统中的低钾（LK）和高钾（HK）绵羊网织红细胞成熟过程中，对钠泵和钠激活的ATP水解（Na⁺-ATP酶）的变化进行了跟踪研究。LK细胞网织红细胞的体外成熟导致细胞内钾离子、哇巴因敏感的⁸⁶Rb⁺摄取量以及Na⁺-ATP酶活性逐渐降低。虽然体外成熟（长达10天）并未导致动力学行为从网织红细胞的典型行为转变为成熟细胞的典型行为，但在体内长期成熟后，动力学变化明显可见。通过比较大量失血后恢复过程中不同时期获得的血液中最轻（最年轻）和最重（最老）细胞组分的Na⁺-ATP酶（对钾离子的反应）特性，证实了HK细胞的这一点。得出的结论是，未成熟细胞和成熟细胞之间的动力学差异是由于细胞成熟所致，并且反映了某些类型泵位点的选择性变化和（或）丢失。
