Weigensberg A M, Blostein R
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4978-82. doi: 10.1073/pnas.80.16.4978.
The effect of metabolic depletion on the maturation-associated loss of membrane functions has been studied by using sheep reticulocytes incubated in vitro at 37 degrees C for periods up to 41 hr. ATP was either maintained with glucose, adenosine plus inosine, or depleted with 2-deoxyglucose plus arsenate. Two membrane transport systems were studied: Na+-dependent glycine transport activity and the sodium pump, estimated from measurements of the number of [3H]ouabain binding sites per cell. Both transport systems were decreased during maturation. However, the decrease was much less in ATP-depleted cells compared to ATP-replete cells. It is concluded that the loss of certain functions during reticulocyte maturation is retarded by metabolic depletion.
通过在37℃体外培养绵羊网织红细胞长达41小时，研究了代谢耗竭对与成熟相关的膜功能丧失的影响。ATP通过葡萄糖、腺苷加肌苷维持，或通过2-脱氧葡萄糖加砷酸盐耗尽。研究了两种膜转运系统：依赖Na + 的甘氨酸转运活性和钠泵，通过测量每个细胞中[3H]哇巴因结合位点的数量来估计。在成熟过程中，两种转运系统均下降。然而，与ATP充足的细胞相比，ATP耗尽的细胞下降幅度要小得多。得出的结论是，网织红细胞成熟过程中某些功能的丧失因代谢耗竭而延迟。
