Bangma G C, ten Donkelaar H J, Pellegrino A
J Comp Neurol. 1983 Apr 10;215(3):258-74. doi: 10.1002/cne.902150303.
In the present study the organization of the corticonuclear projections of the cerebellum in the red-eared turtle Pseudemys scripta elegans was investigated. To find out whether a zonal pattern exists in the cerebellar cortex, a topological analysis was made of the Purkinje cell layer of a Nissl-stained series of the cerebellum. This analysis showed a distinct, longitudinally oriented zonal pattern of Purkinje cells. In each cerebellar half a medial, an intermediate, and a lateral zone could be distinguished. This pattern appeared to correspond in part to an earlier subdivision of the reptilian cerebellum suggested by Larsell ('26, '32, '67). In the experimental part of this study, the corticonuclear projections to the cerebellar nuclei and the vestibular nuclear complex were demonstrated with the horseradish peroxidase (HRP) technique. All projections of the Purkinje cells appeared to be strictly ipsilateral. After HRP slow-release gel implantations at various levels of the vestibular nuclear complex, longitudinally oriented areas of labeled Purkinje cells were found in the lateral part of the medial zone, the intermediate zone, and the lateral zone. Two strips of Purkinje cells remained unlabeled: One in the rostrolateral part of the intermediate zone, and another in the medial part of the medial zone. After HRP gel implantations in the cerebellar peduncle aimed at the medial and lateral cerebellar nuclei, respectively. Purkinje cells in the latter two strips were labeled. The present results indicate that the corticonuclear projections to the medial cerebellar nucleus arise in the medial part of the medial zone and those to the lateral cerebellar nucleus in the rostrolateral part of the intermediate zone. It can be concluded that cerebellar zones as demonstrated in the turtle Pseudemys scripta elegans, are phylogenetically very old and may represent the basic functional circuit of the cerebellum.
在本研究中，对红耳龟（滑龟指名亚种）小脑皮质核投射的组织进行了研究。为了确定小脑皮质中是否存在带状模式，对小脑尼氏染色系列的浦肯野细胞层进行了拓扑分析。该分析显示浦肯野细胞呈现出明显的纵向带状模式。在每个小脑半球中，可以区分出内侧区、中间区和外侧区。这种模式似乎部分对应于拉塞尔（1926年、1932年、1967年）之前提出的爬行动物小脑的细分。在本研究的实验部分，用辣根过氧化物酶（HRP）技术显示了小脑核和前庭核复合体的皮质核投射。浦肯野细胞的所有投射似乎都是严格同侧的。在前庭核复合体的不同水平植入HRP缓释凝胶后，在内侧区外侧部分、中间区和外侧区发现了纵向排列的标记浦肯野细胞区域。有两条浦肯野细胞带未被标记：一条在中间区的 rostrolateral 部分，另一条在内侧区的内侧部分。分别将HRP凝胶植入小脑脚以靶向内侧和外侧小脑核后，后两条带中的浦肯野细胞被标记。目前的结果表明，投射到内侧小脑核的皮质核起源于内侧区的内侧部分，而投射到外侧小脑核的起源于中间区的 rostrolateral 部分。可以得出结论，滑龟指名亚种中显示的小脑区域在系统发育上非常古老，可能代表了小脑的基本功能回路。
