Ficht T A, Moyer R W
J Biol Chem. 1980 Jul 25;255(14):7040-8.
A well defined enzyme comples of approximately 5 X 10(6) daltons that contains phage and host cell components known to be required for the processes of phage transcription and DNA replication has been isolated from bacteriophage T5-infected Escherichia coli cells. In addition to the RNA polymerase of the host cell, the complex contains the phage-encoded: gpC2 which has been implicated genetically as a controlling element of late transcription; gpD9, the DNA polymerase required for T5 DNA replication; the proteins gpD5 (DNA-binding protein), and gpD15 (nuclease) which are both known to be essential for T5 DNA replication and for the initiation of late transcription. The viral gpD5 derived from the purified complex is a phosphoprotein. The enzyme complex also contains, protected from the action of nuclease, double-stranded DNA with an approximate molecular weight of 1 to 2 X 10(6) (2 to 3% of the size of the T5 genome) which is derived preferentially from the center of the T5 DNA molecule. The composition of the enzyme complex suggests that the processes of transcription and replication are integrated in T5-infected cells.
已从噬菌体T5感染的大肠杆菌细胞中分离出一种定义明确的酶复合物，其分子量约为5×10⁶道尔顿，包含噬菌体和宿主细胞的成分，这些成分是噬菌体转录和DNA复制过程所必需的。除宿主细胞的RNA聚合酶外，该复合物还包含噬菌体编码的：gpC2，遗传学上认为它是晚期转录的控制元件；gpD9，T5 DNA复制所需的DNA聚合酶；蛋白质gpD5（DNA结合蛋白）和gpD15（核酸酶），已知它们对T5 DNA复制和晚期转录的起始都是必不可少的。从纯化复合物中获得的病毒gpD5是一种磷蛋白。该酶复合物还含有分子量约为1至2×10⁶（占T5基因组大小的2%至3%）的双链DNA，它受核酸酶作用的保护，且优先来源于T5 DNA分子的中心。酶复合物的组成表明，转录和复制过程在T5感染的细胞中是整合的。
