Major E O
Prog Clin Biol Res. 1983;105:15-27.
Human embryonic kidney cells were transformed at their third passage with an origin-defective mutant of SV40, 1-11. One clone of these cells, SV1, demonstrated several phenotypes of transformation including high-density growth and anchorage independence. The SV1 cells also were 100% T antigen-positive when tested by immunofluorescence and immunoprecipitation assays. These cells with the SV40 T protein were able to support the persistent replication of BK viral DNA but did not support virus multiplication. The SV1 cells would also support the replication of the Sau 3A A fragment of the BK genome cloned into pBR 322. However, SV1 cells would not support multiplication of JC virus or replication of JC DNA.
人胚肾细胞在第三代时用SV40的1-11号原点缺陷突变体进行转化。这些细胞中的一个克隆，即SV1，表现出几种转化表型，包括高密度生长和不依赖贴壁生长。通过免疫荧光和免疫沉淀试验检测，SV1细胞的T抗原也呈100%阳性。这些带有SV40 T蛋白的细胞能够支持BK病毒DNA的持续复制，但不支持病毒增殖。SV1细胞也能支持克隆到pBR 322中的BK基因组Sau 3A A片段的复制。然而，SV1细胞不支持JC病毒的增殖或JC DNA的复制。
