Grinnell B W, Martin J D, Padgett B L, Walker D L
Prog Clin Biol Res. 1983;105:61-77.
We have examined both the naturally occurring and passage-induced variation in the genome of the human polyomavirus, JCV. JCV DNA was extracted directly from diseased brain tissue of ten cases of progressive multifocal leukoencephalopathy (PML) and the DNA population from any one case was homogeneous with respect to length and restriction endonuclease cleavage patterns. However, a comparison of cloned JCV DNAs revealed that the viral DNAs derived from different cases of PML were not identical. We identified a hypervariable region near the origin of DNA replication, and we demonstrated that there are two genetically distinguishable subtypes of the virus. After growth in vitro, the DNA from certain isolates of JCV became heterogeneous in size. Deletions of up to 12% of the genome occurred after as few a three low multiplicity passages in human glial cells. Most of the deletions mapped within the region presumed to code for T antigen. In addition, we examined the physical properties of JCV from brain and, as expected, they were typical of the polyomavirus genus.
我们研究了人类多瘤病毒JCV基因组中自然发生的变异和传代诱导的变异。JCV DNA直接从10例进行性多灶性白质脑病（PML）患者的病变脑组织中提取，任一病例的DNA群体在长度和限制性内切酶切割模式方面都是均一的。然而，对克隆的JCV DNA的比较显示，源自不同PML病例的病毒DNA并不相同。我们在DNA复制起点附近鉴定出一个高变区，并证明该病毒有两种基因上可区分的亚型。在体外培养后，JCV某些分离株的DNA在大小上变得不均一。在人类神经胶质细胞中进行低传代次数少至三次后，基因组发生了高达12%的缺失。大多数缺失位于推测编码T抗原的区域内。此外，我们检测了来自脑组织的JCV的物理特性，不出所料，它们具有多瘤病毒属的典型特征。
