Kean Jaime M, Rao Suchitra, Wang Michael, Garcea Robert L
Department of Microbiology, University of Colorado School of Medicine, Denver, Colorado, USA.
PLoS Pathog. 2009 Mar;5(3):e1000363. doi: 10.1371/journal.ppat.1000363. Epub 2009 Mar 27.
In addition to the previously characterized viruses BK and JC, three new human polyomaviruses (Pys) have been recently identified: KIV, WUV, and Merkel Cell Py (MCV). Using an ELISA employing recombinant VP1 capsid proteins, we have determined the seroprevalence of KIV, WUV, and MCV, along with BKV and JCV, and the monkey viruses SV40 and LPV. Soluble VP1 proteins were used to assess crossreactivity between viruses. We found the seroprevalence (+/- 1%) in healthy adult blood donors (1501) was SV40 (9%), BKV (82%), JCV (39%), LPV (15%), KIV (55%), WUV (69%), MCV strain 350 (25%), and MCV strain 339 (42%). Competition assays detected no sero-crossreactivity between the VP1 proteins of LPV or MCV or between WUV and KIV. There was considerable sero-crossreactivity between SV40 and BKV, and to a lesser extent, between SV40 and JCV VP1 proteins. After correcting for crossreactivity, the SV40 seroprevalence was approximately 2%. The seroprevalence in children under 21 years of age (n = 721) for all Pys was similar to that of the adult population, suggesting that primary exposure to these viruses likely occurs in childhood.
除了之前已被鉴定的BK和JC病毒外，最近还发现了三种新的人类多瘤病毒（Py）：KIV、WUV和默克尔细胞多瘤病毒（MCV）。我们使用一种采用重组VP1衣壳蛋白的酶联免疫吸附测定法（ELISA），测定了KIV、WUV和MCV以及BK病毒（BKV）、JC病毒（JCV）、猴病毒SV40和LPV的血清流行率。可溶性VP1蛋白用于评估病毒之间的交叉反应性。我们发现，健康成年献血者（1501人）中的血清流行率（±1%）分别为：SV40（9%）、BKV（82%）、JCV（39%）、LPV（15%）、KIV（55%）、WUV（69%）、MCV毒株350（25%）和MCV毒株339（42%）。竞争试验未检测到LPV或MCV的VP1蛋白之间或WUV与KIV之间存在血清交叉反应性。SV40与BKV之间存在相当程度的血清交叉反应性，在较小程度上，SV40与JCV的VP1蛋白之间也存在交叉反应性。校正交叉反应性后，SV40的血清流行率约为2%。所有多瘤病毒在21岁以下儿童（n = 721）中的血清流行率与成年人群相似，这表明对这些病毒的初次接触可能发生在儿童期。
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