Augustyniak H, Borsuk P, Hirschler I, Stepień P P, Bartnik E
Gene. 1983 Apr;22(1):69-74. doi: 10.1016/0378-1119(83)90065-3.
Mitochondrial DNA (mtDNA) was isolated from lupine. Restriction analysis was used to estimate its size, which is about 180 kb. A BamHI bank of this mtDNA was constructed using plasmids pBR322 and pBR327 as vectors. Eight clones containing plasmids hybridizing to mitochondrial tRNA (mttRNA) were isolated. Restriction maps of these plasmids were determined. Six of these plasmids hybridized to unique fragments and two to two fragments of very similar size, all obtained by BamHI cleavage of mtDNA.
从羽扇豆中分离出线粒体DNA（mtDNA）。采用限制性分析来估计其大小，约为180 kb。以质粒pBR322和pBR327为载体构建了该mtDNA的BamHI文库。分离出八个含有与线粒体tRNA（mttRNA）杂交的质粒的克隆。确定了这些质粒的限制性图谱。其中六个质粒与独特的片段杂交，两个与大小非常相似的两个片段杂交，所有片段均通过mtDNA的BamHI切割获得。
