Yourist J E, Haines H G, Miller K D
J Gen Virol. 1983 Jul;64 (Pt 7):1475-81. doi: 10.1099/0022-1317-64-7-1475.
A peptide, prepared by gentle oxidative detoxification of a methionine-free cobra alpha-neurotoxin, was evaluated for antiviral activities in vivo and in vitro. When added to BHK cells before infection the peptide reduced herpes simplex virus type 1 (HSV-1)-induced TCD50 in a dose- and time-dependent manner. The antiviral effect was also expressed on initiation of cell treatment 1 h after infection if the peptide was left in contact with cells. Single, intracerebral doses of the toxoid in suckling mice significantly increased 50% survival times, and reduced virus replication in the infected brain tissues. Cutaneous lesions and resultant scar formation induced by HSV-1 in hairless mice were also significantly reduced by subcutaneous peptide injections.
一种通过对不含蛋氨酸的眼镜蛇α-神经毒素进行温和氧化解毒制备的肽，在体内和体外进行了抗病毒活性评估。在感染前添加到BHK细胞中时，该肽以剂量和时间依赖性方式降低了1型单纯疱疹病毒（HSV-1）诱导的半数组织培养感染剂量（TCD50）。如果该肽与细胞接触，在感染后1小时开始细胞处理时也表现出抗病毒作用。在乳鼠中单次脑内注射类毒素剂量显著增加了50%的存活时间，并减少了感染脑组织中的病毒复制。皮下注射肽也显著减少了无毛小鼠中由HSV-1诱导的皮肤损伤和由此产生的瘢痕形成。
