Kelly P M, Shanley J D, Sears J
J Med Virol. 1983;11(3):257-64. doi: 10.1002/jmv.1890110309.
Recently, the biological and biochemical characteristics of EMC virus have received considerable attention, but little is known concerning the relative susceptibility of various mammalian cells to EMC virus. In this study, a variety of mammalian cell types was evaluated for susceptibility to the M strain of EMC virus by comparing peak viral titers, time course of infection, plaquing efficiency, and formation of viral antigens. Infection of all murine and guinea pig cell types resulted in substantial virus replication and induction of viral antigens in greater than 95% of cells. Human cell lines supported replication with a slower time course, but significant increases in viral yield and plaquing efficiency occurred with viral adaptation following successive replication cycles in human cells. All rat cell lines were relatively resistant to EMC virus infection and replication, and less than 1% of rat cells expressed viral antigens. Attempts were unsuccessful to adapt virus by successive replication cycles in rat cells. The possible reasons for the relative resistance of rat cells are currently under investigation.
最近，脑心肌炎病毒的生物学和生化特性受到了相当大的关注，但对于各种哺乳动物细胞对脑心肌炎病毒的相对易感性却知之甚少。在本研究中，通过比较病毒峰值滴度、感染时间进程、空斑形成效率和病毒抗原的形成，评估了多种哺乳动物细胞类型对脑心肌炎病毒M株的易感性。所有小鼠和豚鼠细胞类型的感染都导致了大量病毒复制，并在超过95%的细胞中诱导了病毒抗原。人类细胞系支持病毒复制，但时间进程较慢，不过在人类细胞中连续复制循环后，随着病毒适应性的出现，病毒产量和空斑形成效率显著增加。所有大鼠细胞系对脑心肌炎病毒感染和复制相对耐药，不到1%的大鼠细胞表达病毒抗原。通过在大鼠细胞中连续复制循环来使病毒适应的尝试未成功。大鼠细胞相对耐药的可能原因目前正在研究中。
