Izawa T, Koshimizu E, Komabayashi T, Tsuboi M
Nihon Seirigaku Zasshi. 1983;45(1):36-44.
The effects of Ca2+ and calmodulin inhibitors on lipolysis induced by epinephrine, norepinephrine, caffeine and ACTH in rat epididymal adipose tissue were investigated. 1. Omission of Ca2+ from the incubation medium slightly depressed lipolysis induced by epinephrine, norepinephrine and ACTH. Lipolysis induced by caffeine was significantly depressed. 2. Lipolysis induced by epinephrine, norepinephrine, caffeine and ACTH was strongly depressed when Ca2+-deficient tissue was incubated in Ca2+-free Ringer solution. 3. In Ca2+-deficient tissue, the addition of 0.75mM Ca2+ apparently restored lipolysis induced by epinephrine, norepinephrine and ACTH, whereas that by caffeine was restored to only approximately 89%. 4. The addition of La3+ markedly inhibited lipolysis induced by each agonist. 5. The Ca2+ antagonists such as verapamil and diltiazem dose-dependently inhibited lipolysis induced by each agonist. 6. The specific calmodulin inhibitors such as chlorpromazine, trifluoperazine and W-7 markedly inhibited lipolysis induced by each agonist. These results strongly support the possible key role that the redistribution and influx of Ca2+ may play in lipolysis induced by epinephrine, norepinephrine, caffeine and ACTH, and further suggest that calmodulin may affect lipolysis.
研究了钙离子（Ca2+）和钙调蛋白抑制剂对大鼠附睾脂肪组织中由肾上腺素、去甲肾上腺素、咖啡因和促肾上腺皮质激素（ACTH）诱导的脂肪分解的影响。1. 孵育培养基中去除Ca2+会轻微抑制由肾上腺素、去甲肾上腺素和ACTH诱导的脂肪分解。由咖啡因诱导的脂肪分解则受到显著抑制。2. 当将缺钙组织置于无Ca2+的林格氏液中孵育时，由肾上腺素、去甲肾上腺素、咖啡因和ACTH诱导的脂肪分解受到强烈抑制。3. 在缺钙组织中，添加0.75mM Ca2+明显恢复了由肾上腺素、去甲肾上腺素和ACTH诱导的脂肪分解，而由咖啡因诱导的脂肪分解仅恢复到约89%。4. 添加La3+显著抑制了每种激动剂诱导的脂肪分解。5. 维拉帕米和地尔硫䓬等Ca2+拮抗剂剂量依赖性地抑制了每种激动剂诱导的脂肪分解。6. 氯丙嗪、三氟拉嗪和W-7等特异性钙调蛋白抑制剂显著抑制了每种激动剂诱导的脂肪分解。这些结果有力地支持了Ca2+的重新分布和内流可能在由肾上腺素、去甲肾上腺素、咖啡因和ACTH诱导的脂肪分解中发挥关键作用，并且进一步表明钙调蛋白可能影响脂肪分解。
