Bender M A, Christensen J, Brockman W W
J Virol. 1983 Jul;47(1):115-24. doi: 10.1128/JVI.47.1.115-124.1983.
A transformation revertant has been isolated from an unusual line of simian virus 40 (SV40)-transformed BALB/c-3T3 cells in which rearrangements of integrated viral sequences are common. The revertant produces no SV40 T antigens, yields no virus on fusion with permissive cells, and can be retransformed by SV40 virions. SV40 DNA sequences are present within the cellular DNA, but interruption of the viral early transcription region by deletion and recombination with cellular sequences precludes the synthesis of T antigens. Analysis of this revertant lends further support to the notion that large T antigen plays an essential role in the maintenance of transformation in SV40-transformed BALB/c-3T3 cells. Examination of integration of SV40 DNA in this revertant, as well as in a temperature-sensitive A transformant, after retransformation by SV40 confirms that sequence homology plays little role in the insertion of SV40 DNA into cellular chromosomes.
从一组不寻常的猴病毒40（SV40）转化的BALB/c - 3T3细胞系中分离出了一个转化回复突变体，在该细胞系中整合病毒序列的重排很常见。该回复突变体不产生SV40 T抗原，与允许性细胞融合时不产生病毒，并且可以被SV40病毒粒子重新转化。SV40 DNA序列存在于细胞DNA中，但病毒早期转录区域因与细胞序列的缺失和重组而中断，从而阻止了T抗原的合成。对该回复突变体的分析进一步支持了大T抗原在SV40转化的BALB/c - 3T3细胞中维持转化起关键作用这一观点。在用SV40重新转化后，对该回复突变体以及一个温度敏感A转化体中SV40 DNA整合情况的检测证实，序列同源性在SV40 DNA插入细胞染色体过程中作用不大。
