Ryan K W, Christensen J B, Imperiale M J, Brockman W W
Mol Cell Biol. 1985 Dec;5(12):3577-82. doi: 10.1128/mcb.5.12.3577-3582.1985.
In an attempt to identify cellular genes that might be involved in simian virus 40 (SV40) transformation, we have set out to isolate cells which express T antigen but are not transformed. SV40 DNA and the herpes simplex virus thymidine kinase gene were cotransfected into tk- 3T3 fibroblasts. Of 72 colonies screened that were resistant to hypoxanthine-aminopterin-thymidine, 57 were T antigen positive as judged by immunofluorescence. One of these lines, A27, had a normal growth phenotype in monolayer overgrowth and soft agar assays. It contained intact SV40 sequences that could be rescued by fusion to permissive cells. This rescued virus was fully capable of transforming nonpermissive cells to the same extent as did wild-type virus. The A27 cells, however, were not transformable by infection with SV40 or by transfection of SV40 DNA. It is likely that these cells were altered in a cellular function required for the establishment of the transformed state.
为了鉴定可能参与猿猴病毒40（SV40）转化的细胞基因，我们着手分离那些表达T抗原但未被转化的细胞。将SV40 DNA和单纯疱疹病毒胸苷激酶基因共转染到tk-3T3成纤维细胞中。在筛选的72个对次黄嘌呤-氨基蝶呤-胸腺嘧啶有抗性的菌落中，通过免疫荧光判断有57个T抗原呈阳性。其中一个细胞系A27，在单层过度生长和软琼脂试验中具有正常的生长表型。它含有完整的SV40序列，可通过与允许细胞融合而拯救出来。这种拯救出的病毒完全能够像野生型病毒一样将非允许细胞转化到相同程度。然而，A27细胞不能通过感染SV40或转染SV40 DNA而被转化。这些细胞很可能在建立转化状态所需的细胞功能方面发生了改变。
