猴病毒40的tsA突变体对BALB/c - 3T3细胞的转化：转化技术对转化表型的影响
Transformation of BALB/c-3T3 cells by tsA mutants of simian virus 40: effect of transformation technique on the transformed phenotype.
作者信息
Kelley S, Bender M A, Brockman W W
出版信息
J Virol. 1980 Jan;33(1):550-2. doi: 10.1128/JVI.33.1.550-552.1980.
Abstract
Simian virus 40 tsA-transformed BALB/c-3T3 cells isolated as foci of overgrowth in liquid medium were compared with those isolated as colonies in soft agar. Efficiencies of transformation were equivalent in the two procedures. Cells isolated as foci were able to grow in agar and vice versa. No difference in temperature sensitivity of the transformed phenotype was detected when tsA transformants selected in agar were compared with those selected as foci. The use of the two different transformation procedures, then, did not form the basis for generation of different transformed phenotypes, and transformants generated in both ways were dependent upon expression of the A gene for maintenance of the transformed state.
摘要
将在液体培养基中作为过度生长灶分离得到的猿猴病毒40 tsA转化的BALB/c - 3T3细胞与在软琼脂中作为集落分离得到的细胞进行比较。两种方法的转化效率相当。作为灶分离得到的细胞能够在琼脂中生长，反之亦然。当比较在琼脂中选择的tsA转化体与作为灶选择的转化体时，未检测到转化表型的温度敏感性差异。因此，使用两种不同的转化程序并不是产生不同转化表型的基础，并且通过两种方式产生的转化体都依赖于A基因的表达来维持转化状态。
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本文引用的文献
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AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.用于多瘤病毒转化细胞选择性测定的琼脂悬浮培养
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Conditions leading to the establishment of the N (a gene dependent) and A (a gene independent) transformed states after polyoma virus infection of rat fibroblasts.大鼠成纤维细胞受多瘤病毒感染后导致N（基因依赖性）和A（基因非依赖性）转化状态建立的条件。
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Growth control in simian virus 40-transformed rat cells: temperature-independent expression of the transformed phenotype in tsA transformants derived by agar selection.猿猴病毒40转化大鼠细胞中的生长控制：通过琼脂选择获得的tsA转化体中转化表型的温度非依赖性表达。
J Virol. 1978 Oct;28(1):1-5. doi: 10.1128/JVI.28.1.1-5.1978.
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Transformation of BALB/c-3T3 cells by tsA mutants of simian virus 40: temperature sensitivity of the transformed phenotype and retransofrmation by wild-type virus.猿猴病毒40的tsA突变体对BALB/c - 3T3细胞的转化：转化表型的温度敏感性及野生型病毒的再次转化
J Virol. 1978 Mar;25(3):860-70. doi: 10.1128/JVI.25.3.860-870.1978.
5
Temperature-sensitive growth regulation in one type of transformed rat cells induced by the tsa mutant of polyoma virus.多瘤病毒tsa突变体诱导的一种转化大鼠细胞中的温度敏感生长调节
J Virol. 1977 Dec;24(3):721-8. doi: 10.1128/JVI.24.3.721-728.1977.
6
Initiation and maintenance of cell transformation by simian virus 40: a viral genetic property.猴病毒40引发和维持细胞转化：一种病毒遗传特性。
Proc Natl Acad Sci U S A. 1975 Feb;72(2):673-7. doi: 10.1073/pnas.72.2.673.
7
Simian virus 40 gene A function and maintenance of transformation.猴病毒40基因A的功能与转化维持
J Virol. 1975 Mar;15(3):636-44. doi: 10.1128/JVI.15.3.636-644.1975.
8
Role of simian virus 40 gene A function in maintenance of transformation.猿猴病毒40基因A功能在维持转化中的作用。
J Virol. 1975 Mar;15(3):619-35. doi: 10.1128/JVI.15.3.619-635.1975.
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Function of simian virus 40 gene A in transforming infection.猴病毒40基因A在转化感染中的功能。
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Simian virus 40 functions required for the establishment and maintenance of malignant transformation.猴病毒40在恶性转化的建立和维持中所需的功能。
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