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插入元件IS1在大肠杆菌自然分离株中的分布。

Distribution of insertion element IS1 in natural isolates of Escherichia coli.

作者信息

Nyman K, Ohtsubo H, Davison D, Ohtsubo E

出版信息

Mol Gen Genet. 1983;189(3):516-8. doi: 10.1007/BF00325920.

DOI:10.1007/BF00325920
PMID:6306398
Abstract

Total DNAs from twelve natural isolates of Escherichia coli from animals and humans were examined by hybridization with a probe for IS1. Considerable variation in copy number was found. In the case of two strains isolated from the same individual, one strain contained no copies of IS1 and the other, much greater than 30. Evidence was also obtained for the existence of IS1-like elements (iso-IS1s) of greater than 15% sequence divergence relative to the IS1 from antibiotic resistance plasmid R100.

摘要

用IS1探针通过杂交检测了来自动物和人类的12株大肠杆菌自然分离株的总DNA。发现拷贝数存在相当大的差异。在从同一个体分离出的两株菌株中,一株不含IS1拷贝,而另一株的拷贝数远大于30。还获得了证据,证明存在与来自抗生素抗性质粒R100的IS1序列差异大于15%的类IS1元件(iso-IS1s)。

相似文献

1
Distribution of insertion element IS1 in natural isolates of Escherichia coli.插入元件IS1在大肠杆菌自然分离株中的分布。
Mol Gen Genet. 1983;189(3):516-8. doi: 10.1007/BF00325920.
2
Four types of IS1 with differences in nucleotide sequence reside in the Escherichia coli K-12 chromosome.四种核苷酸序列存在差异的IS1位于大肠杆菌K-12染色体中。
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3
Nonrandom location of IS1 elements in the genomes of natural isolates of Escherichia coli.大肠杆菌自然分离株基因组中IS1元件的非随机定位。
Mol Biol Evol. 1997 Jul;14(7):725-32. doi: 10.1093/oxfordjournals.molbev.a025812.
4
Comparison of IS1, IS2 and IS3 copy number in Escherichia coli strains K-12, B and C.大肠杆菌K-12、B和C菌株中IS1、IS2和IS3拷贝数的比较
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5
Fate of donor insertion sequence IS1 during transposition.
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Analysis of transposable elements inserted in the genomes of bacteriophages Mu and P1.对插入噬菌体Mu和P1基因组中的转座元件的分析。
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7
Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1.决定由插入序列IS1介导的质粒共整合频率的因素。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):277-81. doi: 10.1073/pnas.79.2.277.
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Deletions and an inversion induced by a resident IS1 of the lactose transposon Tn951.乳糖转座子Tn951的一个常驻IS1诱导的缺失和倒位。
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Insertions of transposable elements in the promoter proximal region of the gene cluster for Escherichia coli H+-ATPase: 8 base pair repeat generated by insertion of IS1.转座元件插入大肠杆菌H⁺-ATP酶基因簇启动子近端区域:由IS1插入产生的8碱基对重复序列
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The ancestry of insertion sequences common to Escherichia coli and Salmonella typhimurium.大肠杆菌和鼠伤寒沙门氏菌共有的插入序列的起源。
J Bacteriol. 1993 Dec;175(24):7863-8. doi: 10.1128/jb.175.24.7863-7868.1993.

引用本文的文献

1
Natural populations of Escherichia coli and Salmonella typhimurium harbor the same classes of insertion sequences.大肠杆菌和鼠伤寒沙门氏菌的自然种群含有相同类别的插入序列。
Genetics. 1993 Mar;133(3):449-54. doi: 10.1093/genetics/133.3.449.
2
The ancestry of insertion sequences common to Escherichia coli and Salmonella typhimurium.大肠杆菌和鼠伤寒沙门氏菌共有的插入序列的起源。
J Bacteriol. 1993 Dec;175(24):7863-8. doi: 10.1128/jb.175.24.7863-7868.1993.
3
Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?

本文引用的文献

1
Genetic diversity and structure in Escherichia coli populations.大肠杆菌群体中的遗传多样性与结构
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Isolation of polA mutation that affects transposition of insertion sequences and transposons.影响插入序列和转座子转座的polA突变的分离
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Standard reference strains of Escherichia coli from natural populations.来自自然种群的大肠杆菌标准参考菌株。
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Insertions of transposable elements in the promoter proximal region of the gene cluster for Escherichia coli H+-ATPase: 8 base pair repeat generated by insertion of IS1.转座元件插入大肠杆菌H⁺-ATP酶基因簇启动子近端区域:由IS1插入产生的8碱基对重复序列
Mol Gen Genet. 1984;194(1-2):179-87. doi: 10.1007/BF00383514.
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Proc Natl Acad Sci U S A. 1984 Jul;81(14):4500-4. doi: 10.1073/pnas.81.14.4500.
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Frequency of IS1-mediated molecular events in different members of the family Enterobacteriaceae.肠杆菌科不同成员中IS1介导的分子事件的频率。
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Multiple copies of iso-insertion sequences of IS1 in Shigella dysenteriae chromosome.痢疾志贺氏菌染色体中IS1的异插入序列的多个拷贝。
Nature. 1981 Aug 13;292(5824):640-3. doi: 10.1038/292640a0.
6
Distribution of the insertion sequence IS1 in gram-negative bacteria.插入序列IS1在革兰氏阴性菌中的分布。
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Conservation and variation of nucleotide sequences in Escherichia coli strains isolated from nature.从自然界分离的大肠杆菌菌株中核苷酸序列的保守性与变异性
J Bacteriol. 1980 Nov;144(2):560-8. doi: 10.1128/jb.144.2.560-568.1980.
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Electrophoretic variation in Escherichia coli from natural sources.自然来源大肠杆菌的电泳变异
Science. 1973 Dec 7;182(4116):1024-6. doi: 10.1126/science.182.4116.1024.
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
10
Nucleotide sequence of an insertion element, IS1.插入元件IS1的核苷酸序列。
Proc Natl Acad Sci U S A. 1978 Feb;75(2):615-9. doi: 10.1073/pnas.75.2.615.