Klier A, Parsot C, Rapoport G
Nucleic Acids Res. 1983 Jun 25;11(12):3973-87. doi: 10.1093/nar/11.12.3973.
We have determined the conditions required for in vitro transcription of the cloned chromosomal crystal gene from Bacillus thuringiensis using either the homologous vegetative RNA polymerase or a sporulation specific form of this enzyme. The gene is actively transcribed by the latter enzyme (form II) but not by the vegetative one. Evidence for a specific recognition between the form II enzyme and the promotor site of the crystal gene was obtained by binding experiments. They showed that the binding is increased by the presence of some additional factors, which change the specificity of the vegetative core-enzyme. The sequence of the promoter has been determined and the start-point of the transcription deduced. Two hexanucleotide sequences, TACAAT and CCTACG, centered at - 10 and - 35 bp are present, but are somewhat different from the consensus sequences previously described in other bacilli.
我们已经确定了使用苏云金芽孢杆菌同源的营养型RNA聚合酶或该酶的芽孢形成特异性形式对克隆的染色体晶体基因进行体外转录所需的条件。该基因可被后一种酶（II型）有效转录，但不能被营养型酶转录。通过结合实验获得了II型酶与晶体基因启动子位点之间特异性识别的证据。实验表明，某些其他因子的存在会增加结合，这些因子会改变营养型核心酶的特异性。已经确定了启动子的序列并推导了转录起点。存在两个以-10和-35 bp为中心的六核苷酸序列TACAAT和CCTACG，但与先前在其他芽孢杆菌中描述的共有序列略有不同。
