Podestá E J, Solano A R, Attar R, Sánchez M L, Molina y Vedia L
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3986-90. doi: 10.1073/pnas.80.13.3986.
Antibodies against the lutropin receptor have been obtained by the monoclonal antibody technique. Mice were immunized with luteal membrane from ovaries from pseudopregnant rats, containing high lutropin receptor concentration. Hybridoma cells were obtained by fusing mouse myeloma cells with spleen cells from the immunized animal. Five clones were produced that secreted monoclonal antibodies that specifically inhibited lutropin binding to its receptor in a competitive fashion. Antibodies from three clones were capable of blocking biological response to lutropin (e.g., testosterone production by isolated rat Leydig cells). Antibodies secreted by two other clones, however, were capable of acting as Leydig cell stimulators. Immunofluorescence studies demonstrated the presence of receptor capping which may be associated with receptor-mediated testosterone production. Antagonist antibodies could be transformed into agonist by the addition of a second crosslinking anti-mouse IgG. The discovery of agonist antibodies against the receptor molecule proves that the biological information of the lutropin-receptor complex resides in the receptor and not in the hormone.
通过单克隆抗体技术已获得抗促黄体生成素受体的抗体。用来自假孕大鼠卵巢的黄体膜免疫小鼠，该黄体膜含有高浓度的促黄体生成素受体。通过将小鼠骨髓瘤细胞与免疫动物的脾细胞融合获得杂交瘤细胞。产生了五个克隆，它们分泌的单克隆抗体以竞争方式特异性抑制促黄体生成素与其受体的结合。来自三个克隆的抗体能够阻断对促黄体生成素的生物学反应（例如，分离的大鼠睾丸间质细胞产生睾酮）。然而，另外两个克隆分泌的抗体能够作为睾丸间质细胞刺激剂。免疫荧光研究证明存在受体盖帽现象，这可能与受体介导的睾酮产生有关。通过添加第二种交联抗小鼠IgG，拮抗剂抗体可转化为激动剂。针对受体分子的激动剂抗体的发现证明，促黄体生成素 - 受体复合物的生物学信息存在于受体而非激素中。
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