Solano A R, Cremaschi G, Sánchez M L, Borda E, Sterin-Borda L, Podestá E J
Centro de Investigaciones Endocrinológicas, Hospital General de Niños Dr. Ricardo Gutiérrez, Buenos Aires, Argentina.
Proc Natl Acad Sci U S A. 1988 Jul;85(14):5087-91. doi: 10.1073/pnas.85.14.5087.
Purified IgG from BALB/c mouse anti-C3H serum exerts positive inotropic and chronotropic effects in C3H mouse atria and induces testosterone synthesis in C3H mouse Leydig cells. The effect depends on IgG concentration and can be abolished by beta-adrenergic-receptor and luteinizing hormone-receptor antagonists. IgG interferes with the binding of dihydroalprenolol and luteinizing hormone. Monoclonal antibodies against major histocompatibility complex class I antigens were active on the Leydig cells of C3H and BALB/c mice. There was a parallelism between the effect of each individual monoclonal antibody with specificity for a particular haplotype and the response of the target cell from the strains carrying such haplotypes. These antibodies could precipitate the soluble luteinizing hormone-receptor complex. The results suggested that bound hormone triggers the association of major histocompatibility class I antigen with the receptor, thereby activating the respective target cells.
从BALB/c小鼠抗C3H血清中纯化得到的IgG，对C3H小鼠心房有正性变力和变时作用，并能诱导C3H小鼠睾丸间质细胞合成睾酮。该作用取决于IgG浓度，且可被β-肾上腺素能受体拮抗剂和促黄体生成素受体拮抗剂消除。IgG会干扰二氢阿普洛尔和促黄体生成素的结合。针对主要组织相容性复合体I类抗原的单克隆抗体，对C3H和BALB/c小鼠的睾丸间质细胞有活性。每种对特定单倍型具有特异性的单克隆抗体的作用，与携带此类单倍型的品系中靶细胞的反应之间存在平行关系。这些抗体可沉淀可溶性促黄体生成素受体复合物。结果表明，结合的激素触发主要组织相容性I类抗原与受体的结合，从而激活各自的靶细胞。
